The transcriptome of Nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes.
The transcriptome of Nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes.
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Nacobbus aberrans的转录组揭示了植物寄生线虫定居内寄生的进化过程。
DOI：
10.1093/gbe/evu171
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发表时间：
2014-08-13
影响因子：
3.3
通讯作者：
Jones JT
中科院分区：
文献类型：
--
作者：
Eves-van den Akker S;Lilley CJ;Danchin EG;Rancurel C;Cock PJ;Urwin PE;Jones JT
在线虫门中，植物寄生被假设至少在四种情况下独立出现。最具经济破坏性的植物寄生线虫物种，因此最广泛的研究，是那些饲料，因为他们破坏性地通过宿主根迁移引起坏死病变（迁移性内寄生虫）和那些修改宿主根组织，以创建一个营养库，他们饲料（定居性内寄生虫）。假根结线虫Nacobbus aberrans是唯一已知的物种有迁移内寄生和定居内寄生阶段在其生活史。此外，它的定居阶段似乎具有根结线虫和包囊线虫的特征。我们提出了第一个大规模的遗传资源的任何假根结线虫物种。我们使用RNAseq来描述整个生命周期中所有表达基因的相对丰度变化，以提供对这种线虫在寄生模式之间转换时的生物学的有趣见解。对N.所有群体的植物寄生线虫的aberrans证实了它与孢囊线虫和根结线虫的接近。我们提出了一个转录组范围内的横向基因转移事件和效应补体分析。比较了典型根结线虫和孢囊线虫与N.畸变现象揭示了有趣的相似之处。重要的是，被认为是孢囊线虫或根结线虫“特异性”的基因已经在N。畸变。我们的研究结果提供了一个共同的祖先的特点和植物的线虫定居内寄生的进化见解。
Within the phylum Nematoda, plant-parasitism is hypothesized to have arisen independently on at least four occasions. The most economically damaging plant-parasitic nematode species, and consequently the most widely studied, are those that feed as they migrate destructively through host roots causing necrotic lesions (migratory endoparasites) and those that modify host root tissue to create a nutrient sink from which they feed (sedentary endoparasites). The false root-knot nematode Nacobbus aberrans is the only known species to have both migratory endoparasitic and sedentary endoparasitic stages within its life cycle. Moreover, its sedentary stage appears to have characteristics of both the root-knot and the cyst nematodes. We present the first large-scale genetic resource of any false-root knot nematode species. We use RNAseq to describe relative abundance changes in all expressed genes across the life cycle to provide interesting insights into the biology of this nematode as it transitions between modes of parasitism. A multigene phylogenetic analysis of N. aberrans with respect to plant-parasitic nematodes of all groups confirms its proximity to both cyst and root-knot nematodes. We present a transcriptome-wide analysis of both lateral gene transfer events and the effector complement. Comparing parasitism genes of typical root-knot and cyst nematodes to those of N. aberrans has revealed interesting similarities. Importantly, genes that were believed to be either cyst nematode, or root-knot nematode, “specific” have both been identified in N. aberrans. Our results provide insights into the characteristics of a common ancestor and the evolution of sedentary endoparasitism of plants by nematodes.
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影响因子：
2.4
作者：
Bird DM;Jones JT;Opperman CH;Kikuchi T;Danchin EG
通讯作者：
Danchin EG
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
14.9
作者：
Boratyn GM;Camacho C;Cooper PS;Coulouris G;Fong A;Ma N;Madden TL;Matten WT;McGinnis SD;Merezhuk Y;Raytselis Y;Sayers EW;Tao T;Ye J;Zaretskaya I
通讯作者：
Zaretskaya I
影响因子：
64.8
作者：
Flot, Jean-Francois;Hespeels, Boris;Van Doninck, Karine
通讯作者：
Van Doninck, Karine
影响因子：
56.9
作者：
Gladyshev, Eugene A.;Meselson, Matthew;Arkhipova, Irina R.
通讯作者：
Arkhipova, Irina R.