Heterologously expressed carbonic anhydrase from Bacillus mucilaginosus promoting CaCO3 formation by capturing atmospheric CO2

Heterologously expressed carbonic anhydrase from Bacillus mucilaginosus promoting CaCO3 formation by capturing atmospheric CO2
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粘液芽孢杆菌异源表达的碳酸酐酶通过捕获大气 CO2 促进 CaCO3 形成

DOI:
10.1007/s13146-015-0239-4
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发表时间:
2016-03
影响因子:
1.4
通讯作者:
Lian Bin
Lian Bin
中科院分区:
地球科学4区
文献类型:
--
作者:
Xiao Leilei;Lian Bin

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碳酸酐酶(CA)可以催化CO2的可逆水合。然而,胶质芽孢杆菌的单一CA是否有助于捕获CO2和诱导CaCO3的形成仍然是不明确的。为了回答这个问题,并促进更好地了解CA在生物地质过程中的功能,我们克隆了B的五个CA基因。mucilaginosus转化大肠杆菌形成工程菌。异源表达的CA(CA 4)用于测试其是否可以促进以CaCl 2和CO2为底物的碳酸盐形成。实验结果表明,CA 4可以在相对较短的时间内帮助晶体形成。EDS数据证实这些晶体是CaCO3。这表明二氧化碳可能更容易被CA捕获。此外,有或无CA处理之间的碳酸钙晶体的大小和形态的差异是显着的。这些结果为微生物诱导碳酸盐合成提供了新的见解。
Carbonic anhydrase (CA) can catalyse the reversible hydration of CO2. However, whether the single CA of Bacillus mucilaginosus is helpful to the capture of CO2 and the induction of CaCO3 formation is still ambiguous. To answer that question and foster a better understanding of the function of CAs in bio-geological processes, we cloned five CA genes of B. mucilaginosus into Escherichia coli to form engineering bacteria. Heterologously expressed CA (CA4) was used to test whether or not it can promote carbonate formation with CaCl2 and CO2 as the substrates. Experimental results showed that CA4 can help crystal formation in a relatively short period of time. EDS data confirmed that these crystals are CaCO3. It suggests that CO2 may be more easily captured by CA. Moreover, the differences were significant in both the size and morphology of CaCO3 crystals between treatments with or without CA. The results provide new insights into carbonate-induced synthesis by microorganisms.
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