Measured pulmonary arterial tissue stiffness is highly sensitive to AFM indenter dimensions.
Measured pulmonary arterial tissue stiffness is highly sensitive to AFM indenter dimensions.
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DOI：
10.1016/j.jmbbm.2017.05.039
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发表时间：
2017-10
影响因子：
3.9
通讯作者：
Tschumperlin DJ
中科院分区：
文献类型：
--
作者：
Sicard D;Fredenburgh LE;Tschumperlin DJ
肺组织的力学性质在肺动脉高压等疾病的发展和肺组织的正常功能中具有重要意义。因此，它是至关重要的，以尽可能准确地测量肺组织的微观力学性能，以获得洞察与发育，衰老和疾病过程相关的组织硬度的正常和病理范围。本研究利用原子力显微镜（AFM）微压痕技术测量了人肺小动脉（血管直径小于100 μm）的杨氏模量，并考察了AFM针尖的几何形状和直径、肺组织切片厚度以及施加到样品上的工作力范围对测量模量的影响。我们观察到与使用金字塔形尖锐AFM尖端（20 nm半径）相关的肺血管杨氏模量测量值（一个数量级）显著增加，与两个较大的球形尖端（1和2.5 μm半径）相比，其产生统计学上无法区分的结果。组织切片厚度（范围为10 - 50微米）对测量的弹性模量的影响相对较小（<1倍），但导致最薄切片（10微米）相对于较厚切片（20和50微米）的测量弹性模量显著增加。我们还发现，所测量的弹性模量适度地（再次<1倍）但显著地取决于所施加的力的大小，但仅取决于厚（50微米）而非薄（10微米）的组织切片。综上所述，这些结果表明压头形状/半径对肺动脉组织的测量弹性模量的主要影响，组织厚度和施加的力的影响较小。本研究的结果突出了AFM参数选择的重要性，准确表征肺动脉组织的力学性能，并允许比较文献值的肺血管组织力学性能的AFM测量范围内的压头和压痕参数。
The mechanical properties of pulmonary tissues are important in normal function and the development of diseases such as pulmonary arterial hypertension. Hence it is critical to measure lung tissue micromechanical properties as accurately as possible in order to gain insight into the normal and pathological range of tissue stiffness associated with development, aging and disease processes. In this study, we used atomic force microscopy (AFM) micro-indentation to characterize the Young’s modulus of small human pulmonary arteries (vessel diameter less than 100 μm), and examined the influence of AFM tip geometry and diameter, lung tissue section thickness and the range of working force applied to the sample on the measured modulus. We observed a significant increase of the measured Young’s modulus of pulmonary vessels (one order of magnitude) associated with the use of a pyramidal sharp AFM tips (20 nm radius), compared to two larger spherical tips (1 and 2.5 μm radius) which generated statistically indistinguishable results. The effect of tissue section thickness (ranging from 10 to 50 microns) on the measured elastic modulus was relatively smaller (<1-fold), but resulted in a significant increase in measured elastic modulus for the thinnest sections (10 micron) relative to the thicker (20 and 50 micron) sections. We also found that the measured elastic modulus depends modestly (again <1-fold), but significantly, on the magnitude of force applied, but only on thick (50 micron) and not thin (10 micron) tissue sections. Taken together these results demonstrate a dominant effect of indenter shape/radius on the measured elastic modulus of pulmonary arterial tissues, with lesser effects of tissue thickness and applied force. The results of this study highlight the importance of AFM parameter selection for accurate characterization of pulmonary arterial tissue mechanical properties, and allow for comparison of literature values for lung vessel tissue mechanical properties measured by AFM across a range of indenter and indentation parameters.
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影响因子：
3.4
作者：
Alcaraz, J;Buscemi, L;Navajas, D
通讯作者：
Navajas, D
DOI：
10.1042/cs0550477
发表时间：
1978-01-01
期刊：
CLINICAL SCIENCE AND MOLECULAR MEDICINE
影响因子：
--
作者：
BANKS, J;BOOTH, FVM;LEE, GDJ
通讯作者：
LEE, GDJ
影响因子：
3.3
作者：
FINUCANE, KE;COLEBATCH, HJ
通讯作者：
COLEBATCH, HJ
影响因子：
3.5
作者：
Harris, Andrew R.;Charras, G. T.
通讯作者：
Charras, G. T.
影响因子：
3.7
作者：
Kohn JC;Lampi MC;Reinhart-King CA
通讯作者：
Reinhart-King CA