The Erwinia amylovora avrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in pseudomonas syringae.
The Erwinia amylovora avrRpt2EA gene contributes to virulence on pear and AvrRpt2EA is recognized by Arabidopsis RPS2 when expressed in pseudomonas syringae.
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梨火疫病菌 avrRpt2EA 基因对梨具有毒力，当在丁香假单胞菌中表达时，AvrRpt2EA 会被拟南芥 RPS2 识别。
DOI：
10.1094/mpmi-19-0644
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
G. Sundin
中科院分区：
文献类型：
--
作者：
Youfu Zhao;S. He;G. Sundin
淀粉欧文氏菌是引起苹果、梨等蔷薇科植物坏死性火疫病的毁灭性病原菌。为了鉴定寄主植物侵染过程中诱导的基因，我们鉴定并克隆了一个可能的效应基因avrRpt2EA。翻译的AvrRpt2EA蛋白的推导氨基酸序列与以前在紫红色假单胞菌PV中报道的效应蛋白AvrRpt2同源。番茄。这两种蛋白在功能域上有58%的相似性(70%相似)，但分泌和转位信号域不同。AvrRpt2EA启动子区域包含一个典型的‘HRP盒’，这表明avrRpt2EA受另一种sigma因子HrpL的调控。AvrRpt2EA在所有被测试的淀粉菌菌株中都检测到，但在其他密切相关的欧文氏菌中没有检测到。与野生型相比，avrRpt2EA缺失突变体对未成熟梨果实的系统侵染能力降低，表明avrRpt2EA在其天然寄主上起毒力因子的作用。紫丁香菌的生长。表达avrRpt2EA的番茄DC3000的产量是紫丁香疫霉的10倍。结果表明，avrRpt2EA在拟南芥rps2突变体中对番茄DC3000的致病力有促进作用。番茄DC3000在拟南芥上的致病力与紫丁香叶枯病菌相似。番茄avrRpt2。当avrRpt2EA在紫丁香P.syringae PV中表达时。番茄DC3000在其天然形式中，在拟南芥中诱导出弱的超敏反应(HR)；然而，含有紫丁香P.syringae PV的杂交蛋白。番茄avrRpt2信号序列，当从紫球藻PV表达时。番茄avrRpt2启动子，引起了较强的HR。因此，avrRpt2EA的信号序列和启动子可能单独或联合影响其在紫丁香疫霉中的表达、分泌或转位。番茄DC3000。这些结果表明，avrRpt2EA在拟南芥中表达时，被拟南芥中的RPS2抗病基因所识别。番茄DC3000。结果还表明，尽管不同的病原菌，如淀粉菌和丁香疫霉菌可能含有相似的效应器基因，但这些效应器的表达和分泌可能受到本地病原菌的特定调控。
The enterobacterium Erwinia amylovora is a devastating plant pathogen causing necrotrophic fire blight disease of apple, pear, and other rosaceous plants. In an attempt to identify genes induced during infection of host plants, we identified and cloned a putative effector gene, avrRpt2EA. The deduced amino-acid sequence of the translated AvrRpt2EA protein is homologous to the effector protein AvrRpt2 previously reported in Pseudomonas syringae pv. tomato. These two proteins share 58% identity (70% similarity) in the functional domain; however, the secretion and translocation signal domain varied. The avrRpt2EA promoter region contains a typical 'hrp box,' which suggests that avrRpt2EA is regulated by the alternative sigma factor, HrpL. avrRpt2EA was detected in all E. amylovora strains tested but not in other closely related Erwinia species. An avrRpt2EA deletion mutant was reduced in its ability to cause systemic infection on immature pear fruits as compared with the wild-type strain, indicating that avrRpt2EA acts as a virulence factor on its native host. Growth of P. syringae pv. tomato DC3000 expressing avrRpt2EA was 10-fold higher than that of P. syringae pv. tomato DC3000 in an Arabidopsis rps2 mutant, indicating that avrRpt2EA promotes virulence of P. syringae pv. tomato DC3000 on Arabidopsis similar to P. syringae pv. tomato avrRpt2. When avrRpt2EA was expressed in P. syringae pv. tomato DC3000 in its native form, a weak hypersensitive response (HR) was induced in Arabidopsis; however, a hybrid protein containing the P. syringae pv. tomato avrRpt2 signal sequence, when expressed from the P syringae pv. tomato avrRpt2 promoter, caused a strong HR. Thus, the signal sequence and promoter of avrRpt2EA may affect its expression, secretion, or translocation, singly or in combination, in P. syringae pv. tomato DC3000. These results indicated that avrRpt2EA is genetically recognized by the RPS2 disease resistance gene in Arabidopsis when expressed in P. syringae pv. tomato DC3000. The results also suggested that although distinct pathogens such as E. amylovora and P. syringae may contain similar effector genes, expression and secretion of these effectors can be under specific regulation by the native pathogen.
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DOI：
10.1073/pnas.0409468102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Chisholm, ST;Dahlbeck, D;Staskawicz, BJ
通讯作者：
Staskawicz, BJ
DOI：
10.1073/pnas.97.16.8770
发表时间：
2000-08-01
影响因子：
11.1
作者：
Collmer, A;Badel, JL;Alfano, JR
通讯作者：
Alfano, JR
影响因子：
3.5
作者：
Guttman, DS;Greenberg, JT
通讯作者：
Greenberg, JT
影响因子：
11.6
作者：
KUNKEL, BN;BENT, AF;STASKAWICZ, BJ
通讯作者：
STASKAWICZ, BJ