Using diffusion tensor imaging and immunofluorescent assay to evaluate the pathology of multiple sclerosis.
Using diffusion tensor imaging and immunofluorescent assay to evaluate the pathology of multiple sclerosis.
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DOI：
10.1002/jmri.22502
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发表时间：
2011-03
影响因子：
4.4
通讯作者：
Rose, John W.
中科院分区：
文献类型：
--
作者：
Zollinger, Lauren V.;Kim, Tae Ho;Hill, Kenneth;Jeong, Eun K.;Rose, John W.
目的：确定免疫荧光分析(IFA)评估的主要DTI指标预测潜在组织病理学的能力。对1例有多发性硬化(MS)病史的人颈髓(CSC)进行常规T2和3D多次激发-弥散加权平面成像(3DMS-DWEPI)。在白质内选择170个感兴趣区，将其分为高信号病变(HIL)、低信号病变(LIL)和正常外观白质(NAWM)。计算各感兴趣区的纵向扩散系数(λL)、径向扩散系数(λr)和各向异性分数(FA)。然后用针对髓鞘和神经丝染色的抗体进行免疫荧光分析，评估潜在的组织病理学。λ、L和λ-r均值在HIL组明显高于NAWM组和LIL组。IFA对HIL的分析显示有明显的脱髓鞘，没有明显的轴突丢失。HIL和LILS的FA值明显降低。λL增高者FA值降低，λr值相对正常者降低。λ、λr和FA相对于正常值的异常是脱髓鞘的强烈指标。DTI指数不是轴突丢失的特异性指标。IFA分析是在体外环境下显示髓鞘和轴突病理的可靠方法。
To determine the ability of the principal DTI indices to predict the underlying histopathology evaluated with immunofluorescent assay (IFA). Conventional T2 and 3D multishot-diffusion weight echoplanar imaging (3D ms-DWEPI) was performed on a fixed, ex vivo human cervical spinal cord (CSC) from a patient with history of multiple sclerosis (MS). 170 regions of interest were selected within the white matter and categorized as a high intensity lesion (HIL), low intensity lesion (LIL), and normal appearing white matter (NAWM). The longitudinal diffusivity (λl), radial diffusivity (λr), and fractional anisotropy (FA) were obtained from each ROI. The underlying histopathology was then evaluated using immunofluorescent assay with antibodies directed to myelin and neurofilament staining. The mean values for λl, and λr, were significantly elevated within HIL relative to NAWM, and LIL. IFA analysis of HIL demonstrated significant demyelination, without significant if any axon loss. The FA values were significantly reduced in HIL, and LILs. FA values were also reduced in lesions with increased λl, and λr values relative to normal. Aberrant λl, λr, and FA relative to normal values are strong indicators of demyelination. DTI indices are not specific for axon loss. IFA analysis is a reliable method to demonstrate myelin and axon pathology within the ex vivo setting.
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影响因子：
14.5
作者：
DeLuca, G. C.;Williams, K.;Esiri, M. M.
通讯作者：
Esiri, M. M.
影响因子：
2.9
作者：
Kim, Joong Hee;Trinkaus, Kathryn;Song, Sheng-Kwei
通讯作者：
Song, Sheng-Kwei
影响因子：
2.8
作者：
NAGAO, M;OGAWA, M;YAMAUCHI, H
通讯作者：
YAMAUCHI, H
影响因子：
14.5
作者：
Filippi, M;Bozzali, M;Falini, A
通讯作者：
Falini, A
影响因子：
158.5
作者：
Trapp, BD;Peterson, J;Bö, L
通讯作者：
Bö, L