Inductive, scaffold-based, regenerative medicine approach to reconstruction of the temporomandibular joint disk.
Inductive, scaffold-based, regenerative medicine approach to reconstruction of the temporomandibular joint disk.
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DOI：
10.1016/j.joms.2011.12.030
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Brown BN;Chung WL;Almarza AJ;Pavlick MD;Reppas SN;Ochs MW;Russell AJ;Badylak SF
研究了一种由细胞外基质(ECM)组成的装置作为体内诱导模板用于重建腰椎间盘摘除后的TMJ盘。由猪提取的ECM组成的支架材料被配置为模拟TMJ的形状和大小。将该装置植入犬双侧TMJ摘除模型中。椎间盘摘除后，一侧用ECM支架材料修复，另一侧空白作为对照。在植入后6个月，打开关节间隙，评估关节是否有大体病理退行性变化的迹象，并切除新形成的组织进行组织学、生化和生物力学分析。结果表明，植入一种最初的脱细胞材料支持形成与天然TMJ盘相似的部位合适的、具有功能的宿主组织。此外，这还防止了颞窝和下颌骨的严重退行性改变。对侧对照组未见组织形成和轻度至重度大体病理改变。这些结果表明，基于ECM的生物支架可能是一种替代TMJ椎间盘的现成解决方案。
A device composed of extracellular matrix (ECM) was investigated as an inductive template in vivo for reconstruction of the TMJ disk following discectomy. A scaffold material composed of porcine derived ECM was configured to mimic the shape and size of the TMJ. This device was implanted in a canine model of bilateral TMJ discectomy. Following discectomy, one side was repaired with an ECM scaffold material and the contralateral side was left empty as a control. At 6 months post-implantation the joint space was opened, the joints evaluated for signs of gross pathologic degenerative changes, and newly formed tissue was excised for histologic, biochemical, and biomechanical analysis. The results show that implantation of an initially acellular material supported the formation of site-appropriate, functional host tissue which resembled that of the native TMJ disk. Further, this prevented gross degenerative changes in the temporal fossa and mandibular condyle. No tissue formation and mild to severe gross pathologic changes were observed in the contralateral controls. These results suggest that an ECM based bioscaffold may represent an off-the-shelf solution for TMJ disk replacement.
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影响因子：
14
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
DOI：
10.1073/pnas.0905851106
发表时间：
2010-02-23
影响因子：
11.1
作者：
Agrawal, Vineet;Johnson, Scott A.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
6.1
作者：
Bellon, Teresa;Martinez, Virginia;Auxiliadora Bajo, Maria
通讯作者：
Auxiliadora Bajo, Maria
影响因子：
1.9
作者：
Dimitroulis, G
通讯作者：
Dimitroulis, G
影响因子：
1.9
作者：
Dimitroulis, George
通讯作者：
Dimitroulis, George