Surface characterization of extracellular matrix scaffolds.
Surface characterization of extracellular matrix scaffolds.
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DOI：
10.1016/j.biomaterials.2009.09.061
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发表时间：
2010-01
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
关键词：
由不同组织来源或使用不同方法制备的细胞外基质（ECM）支架已被证明对细胞粘附模式以及支持和维持分化表型的能力具有独特的作用。目前尚不清楚ECM的分子组成或超微结构是否在决定与之接触的细胞的表型中起更大的作用。然而，当植入时，材料的拓扑结构和配体景观将决定结合的宿主分子以及介导宿主反应的细胞的类型和行为。因此，全面了解表面特性对于设计用于特定临床应用的支架至关重要。研究了猪膀胱、小肠和肝脏ECM支架的表面特性以及两种常用的化学交联方法对UBM的影响。电子显微镜和飞行时间二次离子质谱被用来检查支架的表面特性。结果表明，ECM支架具有独特的形态和结构特性，这取决于从其中收获支架的器官或组织。此外，结果表明，ECM支架的表面特性通过化学交联而改变。
Extracellular matrix (ECM) scaffolds prepared from different tissue sources or using different methods have been demonstrated to have distinctive effects upon cell adhesion patterns and the ability to support and maintain differentiated phenotypes. It is unknown whether the molecular composition or the ultrastructure of the ECM plays a greater role in determining the phenotype of the cells with which it comes into contact. However, when implanted, the topology and ligand landscape of the material will determine the host molecules that bind and the type and behavior of cells that mediate the host response. Therefore, a comprehensive understanding of surface characteristics is essential in the design of scaffolds for specific clinical applications. The surface characteristics of ECM scaffolds derived from porcine urinary bladder, small intestine, and liver as well as the effects of two commonly used methods of chemical cross-linking upon UBM were investigated. Electron microscopy and time of flight secondary ion mass spectroscopy were used to examine the surface characteristics of the scaffolds. The results show that ECM scaffolds have unique morphologic and structural properties which are dependant on the organ or tissue from which the scaffold is harvested. Furthermore, the results show that the surface characteristics of an ECM scaffold are changed through chemical cross-linking.
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影响因子：
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影响因子：
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通讯作者：
Rundell, AE