Detection of quantitative trait loci for carcass traits in the pig by using AFLP
Detection of quantitative trait loci for carcass traits in the pig by using AFLP
复制标题
利用 AFLP 检测猪胴体性状数量性状位点
作者：
K. Wimmers;E. Muráni;S. Ponsuksili;M. Yerle;K. Schellander
为了评价扩增片段长度多态(AFLP)技术在家畜数量性状基因座检测中的适用性，将AFLP与选择性基因分型相结合，作为标记-QTL关联的快速筛选方法。以影响眼肌面积的基因座为研究对象，从杜洛克×柏林小型猪F2实验杂交组合中筛选出6对极不协调的同胞组合，利用48对AFLP引物组合进行检测。将两个显著的AFLP标记转化为简单的共显性PCR标记(STS-Bo1和STS-Bo3)，并通过物理定位和连锁定位将其归属于Sscr4。单标记分析表明，STS标记与影响眼肌面积的可能的QTL相关。区间作图证实了在Sscr4上存在一个对眼肌面积有显著影响的QTL(Pgenomewide<；0.01)，STS-Bo1是较接近的标记。在同一地点，对屠体长度和背脂厚度也有显著的影响(P<0.05)。我们的结果证明了AFLP分析和选择性基因分型相结合的方法作为一种检测包含QTL的家畜基因组区域的方法是可行的。
For evaluation of the suitability of Amplified Fragment Length Polymorphism (AFLP) for detection of quantitative trait loci in farm animals, a combination of AFLP and selective genotyping has been applied as a rapid screening method for marker–QTL associations. Focusing on loci affecting eye muscle area, six extreme discordant sib pairs were selected from a Duroc × Berlin Miniature Pig F2 experimental cross and examined by using 48 AFLP primer combinations. Two prominent AFLP markers were converted into simple codominant PCR markers (STS-Bo1 and STS-Bo3) and assigned to Sscr4 by physical and linkage mapping. Single marker analysis indicated association of the STS markers with a putative QTL influencing eye muscle area. Interval mapping confirmed the presence of a significant QTL for eye muscle area (Pgenomewide < 0.01) on the Sscr4, with STS-Bo1 being the closer marker. At the same location, significant effects (Pgenomewide < 0.01) on carcass length and backfat thickness were also detected. Our results demonstrate the capability of the combination of AFLP analysis and selective genotyping as a method for detection of genome regions containing QTL in livestock.
影响因子：
56.9
作者：
RISCH, N;ZHANG, HP
通讯作者：
ZHANG, HP