A cell-type-specific atlas of the inner ear transcriptional response to acoustic trauma.
A cell-type-specific atlas of the inner ear transcriptional response to acoustic trauma.
复制标题
内耳对声创伤的转录反应的细胞类型特异性图谱。
DOI：
10.1016/j.celrep.2021.109758
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发表时间：
2021-09-28
期刊：
影响因子：
8.8
通讯作者：
Hertzano R
中科院分区：
文献类型：
--
作者：
Milon B;Shulman ED;So KS;Cederroth CR;Lipford EL;Sperber M;Sellon JB;Sarlus H;Pregernig G;Shuster B;Song Y;Mitra S;Orvis J;Margulies Z;Ogawa Y;Shults C;Depireux DA;Palermo AT;Canlon B;Burns J;Elkon R;Hertzano R
Noise-induced hearing loss (NIHL) results from a complex interplay of damage to the sensory cells of the inner ear, dysfunction of its lateral wall, axonal retraction of type 1C spiral ganglion neurons, and activation of the immune response. We use RiboTag and single-cell RNA sequencing to survey the cell-type-specific molecular landscape of the mouse inner ear before and after noise trauma. We identify induction of the transcription factors STAT3 and IRF7 and immune-related genes across all cell-types. Yet, cell-type-specific transcriptomic changes dominate the response. The ATF3/ATF4 stress-response pathway is robustly induced in the type 1A noise-resilient neurons, potassium transport genes are downregulated in the lateral wall, mRNA metabolism genes are downregulated in outer hair cells, and deafness-associated genes are downregulated in most cell types. This transcriptomic resource is available via the Gene Expression Analysis Resource (gEAR; https://umgear.org/NIHL) and provides a blueprint for the rational development of drugs to prevent and treat NIHL. Milon et al. show that cell-type-specific transcriptomic changes following noise exposure dominate the response compared to common changes. The noise-resilient type 1A neurons induce the ATF3/ATF4 stress-response pathway, and the outer hair cells and lateral wall downregulate mRNA metabolism genes and potassium transport genes, respectively.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
1.5
作者：
Fang, Jie;Zhang, Wen-Cheng;Yamashita, Tetsuji;Gao, Jiangang;Zhu, Min-Sheng;Zuo, Jian
通讯作者：
Zuo, Jian
影响因子：
46.9
作者：
Cadwell CR;Palasantza A;Jiang X;Berens P;Deng Q;Yilmaz M;Reimer J;Shen S;Bethge M;Tolias KF;Sandberg R;Tolias AS
通讯作者：
Tolias AS
影响因子：
15.9
作者：
Fernandez, Katharine A.;Allen, Paul;Cunningham, Lisa L.
通讯作者：
Cunningham, Lisa L.
影响因子：
5.3
作者：
Amici, SA;Dunn, WA;Notterpek, L
通讯作者：
Notterpek, L