Genomic and phenotypic evolution of nematode-infecting microsporidia.
Genomic and phenotypic evolution of nematode-infecting microsporidia.
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DOI：
10.1371/journal.ppat.1011510
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发表时间：
2023-07
期刊：
影响因子：
6.7
通讯作者：
Reinke, Aaron E.
中科院分区：
文献类型：
--
作者：
Wadi, Lina;El Jarkass, Hala Tamim E.;Tran, Tuan;Islah, Nizar E.;Luallen, Robert;Reinke, Aaron E.
微孢子虫是细胞内寄生虫的一大门，可以感染大多数类型的动物。杀线虫属中的许多种都能感染线虫，秀丽隐杆线虫也是研究微孢子虫感染机制的重要模型。为了了解感染线虫的微孢子虫的基因组特性和进化，我们对9种微孢子虫的基因组进行了测序，包括两个属，Enteropsectra和Pancytospora，而没有任何先前测序的基因组。核心细胞过程，包括代谢途径，大多是保守的线虫感染微孢子虫属。每个物种编码属于大基因家族的独特蛋白质，这些蛋白质可能用于与宿主细胞相互作用。最引人注目的是，我们观察到一个这样的家庭，NemLGF 1，是目前在杀线虫和泛胞孢子虫物种，但没有任何其他微孢子虫。为了了解如何杀线虫表型性状的演变，我们测量了宿主范围，组织特异性，孢子大小，和极管长度的属中的几个物种。我们的系统发育分析表明，杀线虫是由两组具有不同特征的物种，并与较长的极管感染多种组织的物种。总之，我们的工作详细介绍了相关微孢子虫物种之间的基因组和性状进化，并为进一步了解微孢子虫进化和感染机制提供了有用的资源。微孢子虫是一种微生物寄生虫，可以感染许多动物。线虫已经成为研究微孢子虫的有用系统，因为这些动物通常被微孢子虫感染，并且这些感染可以在实验室环境中容易地进行研究。为了更好地了解微孢子虫如何进化并在感染不同宿主时改变其特性，我们对感染线虫的9种微孢子虫的基因组进行了测序。我们发现，这些物种的不同分支之间的代谢途径大多是保守的。令人惊讶的是，我们发现了一个家族的蛋白质预测，以促进主机的相互作用，是存在于两个不同的属。并对微孢子虫的形态学特征、感染宿主和组织进行了研究。我们发现，这些属性是相当可变的相关微孢子虫物种。我们的研究结果可以深入了解微孢子虫的进化，并为研究线虫中的微孢子虫感染提供了资源。
Microsporidia are a large phylum of intracellular parasites that can infect most types of animals. Species in the Nematocida genus can infect nematodes including Caenorhabditis elegans, which has become an important model to study mechanisms of microsporidia infection. To understand the genomic properties and evolution of nematode-infecting microsporidia, we sequenced the genomes of nine species of microsporidia, including two genera, Enteropsectra and Pancytospora, without any previously sequenced genomes. Core cellular processes, including metabolic pathways, are mostly conserved across genera of nematode-infecting microsporidia. Each species encodes unique proteins belonging to large gene families that are likely used to interact with host cells. Most strikingly, we observed one such family, NemLGF1, is present in both Nematocida and Pancytospora species, but not any other microsporidia. To understand how Nematocida phenotypic traits evolved, we measured the host range, tissue specificity, spore size, and polar tube length of several species in the genus. Our phylogenetic analysis shows that Nematocida is composed of two groups of species with distinct traits and that species with longer polar tubes infect multiple tissues. Together, our work details both genomic and trait evolution between related microsporidia species and provides a useful resource for further understanding microsporidia evolution and infection mechanisms. Microsporidia are microbial parasites that can infect many animals. Nematodes have become a useful system to study microsporidia as these animals are commonly infected by microsporidia and these infections can be easily studied in a laboratory environment. To better understand how microsporidia evolve and change their properties as they infect different hosts, we sequenced the genomes of nine microsporidia species which infect nematodes. We found that metabolic pathways were mostly conserved between different clades of these species. Surprisingly, we found a family of proteins predicted to facilitate host interactions that is present in two distinct genera. We also determined the hosts and tissues that these microsporidia can infect as well as morphological properties of the microsporidia. We show that these properties are quite variable in related microsporidia species. Our results allow insight into the evolution of microsporidia and provide a resource for studying microsporidia infections in nematodes.
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DOI：
10.1073/pnas.1410442111
发表时间：
2014-10-28
影响因子：
11.1
作者：
Haag, Karen L.;James, Timothy Y.;Ebert, Dieter
通讯作者：
Ebert, Dieter
影响因子：
2.9
作者：
Illergard, Kristoffer;Ardell, David H.;Elofison, Arne
通讯作者：
Elofison, Arne
影响因子：
9.8
作者：
Ehrenbolger K;Jespersen N;Sharma H;Sokolova YY;Tokarev YS;Vossbrinck CR;Barandun J
通讯作者：
Barandun J
影响因子：
3.3
作者：
Dos Santos, Anne Caroline Mascarenhas;Julian, Alexander Thomas;Pombert, Jean-Francois
通讯作者：
Pombert, Jean-Francois
影响因子：
2.2
作者：
de Albuquerque, Nathalia R. M.;Haag, Karen L.
通讯作者：
Haag, Karen L.