OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.).
OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.).
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OsMOGS 是水稻 (Oryza sativa L.) N-聚糖形成和生长素介导的根部发育所必需的
DOI：
10.1111/tpj.12497
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Qi Y
中科院分区：
文献类型：
--
作者：
Wang S;Xu Y;Li Z;Zhang S;Lim JM;Lee KO;Li C;Qian Q;Jiang A;Qi Y
N-糖基化是真核细胞中糖蛋白的主要修饰。在拟南芥中，N-聚糖的功能研究已经取得了很大的进展，但在单子叶植物中的研究很少。本文以水稻（Oryza sativa L.）从拟南芥中分离到一个编码甘露糖寡糖葡萄糖苷酶（OsMOGS）的基因，OsMOGS是拟南芥中α-葡萄糖苷酶I的直向同源物，可修剪内质网中新生肽寡糖链的末端葡萄糖残基。OsMOGS在快速细胞分裂的组织中强烈表达，并且OsMOGS蛋白定位于ER中。OsMOGS的突变完全阻断了N-聚糖的成熟并抑制了高甘露糖N-聚糖的形成。该突变体在根细胞分裂和伸长方面表现出严重缺陷，导致短根表型。此外，由于纤维素合成的减少，根表细胞壁的厚度降低，根毛的形成和伸长受损。进一步的分析表明，由于ATP结合盒转运蛋白（ABCB）的B亚家族的不完全N-糖基化，降低了拟南芥根中生长素的含量和极性运输。我们的研究结果表明OsMOGS参与N-聚糖的形成是生长素介导的水稻根发育所必需的。
N-glycosylation is a major modification of glycoproteins in eukaryotic cells. In Arabidopsis, great progress has been made in functional analysis of N-glycan production; however, there are few studies in monocotyledons. Here, we characterized a rice (Oryza sativa L.) osmogs mutant with shortened roots and isolated a gene coding a putative mannosyl-oligosaccharide glucosidase (OsMOGS), an ortholog of α-glucosidase I in Arabidopsis, which trims the terminal glucosyl residue of the oligosaccharide chain of nascent peptides in the endoplasmic reticulum (ER). OsMOGS is strongly expressed in rapidly cell-dividing tissues and OsMOGS protein is localized in the ER. Mutation of OsMOGS entirely blocked N-glycan maturation and inhibited high-mannose N-glycan formation. The osmogs mutant exhibited severe defects in root cell division and elongation, resulting in a short-root phenotype. In addition, osmogs plants had impaired root hair formation and elongation, and reduced root epidemic cell wall thickness due to decreased cellulose synthesis. Further analysis showed that auxin content and polar transport in osmogs roots were reduced due to incomplete N-glycosylation of the B subfamily of ATP-binding cassette transporter proteins (ABCBs). Our results demonstrate that involvement of OsMOGS in N-glycan formation is required for auxin-mediated root development in rice.
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影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
通讯作者：
Doerner, P
影响因子：
11.6
作者：
Dai, Y;Wang, HZ;Li, JY
通讯作者：
Li, JY
DOI：
10.1074/jbc.m113.475558
发表时间：
2013-08-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Liebminger E;Grass J;Altmann F;Mach L;Strasser R
通讯作者：
Strasser R
DOI：
10.1073/pnas.0800237105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Kang, Jae Sook;Frank, Julia;Koiwa, Hisashi
通讯作者：
Koiwa, Hisashi
影响因子：
7.2
作者：
Chen, SY;Jin, WZ;Wu, P
通讯作者：
Wu, P