Advax™, a polysaccharide adjuvant derived from delta inulin, provides improved influenza vaccine protection through broad-based enhancement of adaptive immune responses.
Advax™, a polysaccharide adjuvant derived from delta inulin, provides improved influenza vaccine protection through broad-based enhancement of adaptive immune responses.
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DOI：
10.1016/j.vaccine.2012.06.021
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发表时间：
2012-08-03
期刊：
影响因子：
5.5
通讯作者：
Petrovsky N
中科院分区：
文献类型：
--
作者：
Honda-Okubo Y;Saade F;Petrovsky N
Advax™佐剂来源于菊粉，菊粉是一种天然植物来源的多糖，当以δ多晶型形式结晶时，具有免疫活性。进行本研究以评估Advax™佐剂增强流感疫苗免疫原性和保护的能力。用单独的流感疫苗或与Advax™佐剂组合的流感疫苗免疫小鼠。进行了抗流感反应的免疫表型分析，包括抗体同种型、B细胞ELISPOT、CD 4和CD 8 T细胞增殖、流感刺激的细胞因子分泌、DTH皮肤试验和活流感病毒激发。Advax™佐剂增加对流感的中和抗体和记忆B细胞应答。它同样增强了CD 4和CD 8 T细胞增殖，并增加了流感刺激的IL-2、IFN-γ、IL-5、IL-6和GM-CSF应答。这转化为对小鼠死亡率和发病率的增强保护。与单独的流感抗原相比，Advax™佐剂提供了显著的抗原剂量节省。免疫血清可将保护从已接受Advax™佐剂疫苗的小鼠转移至未处理小鼠。免疫后12个月，Advax™配制疫苗诱导的增强的体液和T细胞应答持续存在。Advax™佐剂的反应原性较低，未发现不良事件。这表明Advax™佐剂可以是有用的流感疫苗佐剂。
Advax™ adjuvant is derived from inulin, a natural plant-derived polysaccharide that when crystallized in the delta polymorphic form, becomes immunologically active. This study was performed to assess the ability of Advax™ adjuvant to enhance influenza vaccine immunogenicity and protection. Mice were immunized with influenza vaccine alone or combined with Advax™ adjuvant. Immuno-phenotyping of the anti-influenza response was performed including antibody isotypes, B-cell ELISPOT, CD4 and CD8 T-cell proliferation, influenza-stimulated cytokine secretion, DTH skin tests and challenge with live influenza virus. Advax™ adjuvant increased neutralizing antibody and memory B-cell responses to influenza. It similarly enhanced CD4 and CD8 T-cell proliferation and increased influenza-stimulated IL-2, IFN-γ, IL-5, IL-6, and GM-CSF responses. This translated into enhanced protection against mortality and morbidity in mice. Advax™ adjuvant provided significant antigen dose-sparing compared to influenza antigen alone. Protection could be transferred from mice that had received Advax™-adjuvanted vaccine to naïve mice by immune serum. Enhanced humoral and T-cell responses induced by Advax™-formulated vaccine were sustained 12 months post-immunization. Advax™ adjuvant had low reactogenicity and no adverse events were identified. This suggests Advax™ adjuvant could be a useful influenza vaccine adjuvant.
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