Decreased synthesis and increased intracellular degradation of newly synthesized collagen in freshly isolated chick tendon cells incubated with monensin.
Decreased synthesis and increased intracellular degradation of newly synthesized collagen in freshly isolated chick tendon cells incubated with monensin.
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在与莫能菌素孵育的新鲜分离的鸡腱细胞中，新合成的胶原蛋白的合成减少并增加细胞内降解。
DOI：
10.1021/bi00368a056
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Berg,RA
中科院分区：
文献类型：
--
作者：
Neblock,DS;Berg,RA
摘要：羧基离子载体莫能菌素可抑制悬浮培养的鸡胚肌腱成纤维细胞前胶原的合成和分泌。前胶原的合成在暴露于0.1 μ M莫能菌素2小时中被抑制50%，在暴露于0.1 μ M莫能菌素6小时中被抑制70%。在0.1 μ M莫能菌素处理的培养物中，前胶原的分泌被抑制> 90%，并且被更高剂量的试剂完全抑制。在对照细胞中证实了胶原酶可消化肽的细胞库，其水平在莫能菌素处理的培养物中升高3-4倍。为了确定由莫能菌素引起的分泌和合成阻断是否抑制新合成的胶原的细胞内降解，测定对照和莫能菌素处理的培养物中存在的降解的胶原片段中的羟基[14 C]脯氨酸，并与每种培养物中合成的总羟基[14 C]脯氨酸进行比较。新合成的脉冲标记胶原蛋白的细胞内降解速率与对照培养物中的降解速率相当。莫能菌素处理的细胞降解脉冲标记的新合成的胶原蛋白的时间几乎是对照组的两倍，导致新合成的胶原蛋白降解部分的总体增加。结果表明，抑制前胶原的合成和阻断其分泌并不能抑制新合成的胶原的显著部分的细胞内降解。结果还表明，细胞内降解是不依赖于持续的合成，也不需要参与的莫能菌素块远端的分泌途径的元素。新合成的胶原蛋白的细胞内降解是一种现象，现已在多种胶原蛋白产生细胞系和组织中描述（Rennard等人，1982; Bienkowski，1984 a，B;贝格，1986）。已经表明，所有新合成的胶原蛋白中的10-20%被积极参与其合成的细胞降解，并且这种降解发生在分泌之前。
Revised Manuscript Received April 28, 1986 abstract: The synthesis and secretion of procollagen in embryonic chick tendon fibroblasts in suspension culture were inhibited with the carboxylic ionophore monensin. The synthesis of procollagen was inhibited by 50% in a 2-h exposure to 0.1/uM monensin and was inhibited by 70% in a 6-h exposure to 0.1/uM monensin. Secretion of procollagen was inhibited by> 90% in the 0.1/uM monensin-treated cultures and was totally inhibited by higher doses of the reagent. A cellular pool of collagenase-digestible peptides was demonstrated in the control cells, the level of which was elevated 3-4 times in the monensin-treated cultures. In orderto determine whether the secretory and synthesis block caused by monensin inhibited intracellular degradation of newly synthesized collagen, the hydroxy [’4C] proline in degraded collagen fragments present in control and monensin-treated cultures was determined and compared to the totalhydroxy [I4C] proline synthesized in each culture. The intracellular degradation of newlysynthesized, pulse-labeled collagen was shown to proceed at rates comparable to those seen in the control cultures. The monensin-treated cells degraded pulse-labeled newly synthesized collagen nearly twice as long as the controls, resulting in an overall increase in the fraction of newly synthesized collagen that was degraded. The results indicate that inhibiting the synthesis and blocking the secretion of procollagen did not inhibit the intracellular degradation of a significant fraction of newly synthesized collagen. The results also indicate that intracellular degradation is not dependent on continuing synthesis nor does it require the participation of elements of the secretory pathway distal to the monensin block. e intracellular degradation of newlysynthesized collagen is a phenomenon that has now been describedin a variety of collagen-producing cell lines and tissues (Rennard et al., 1982; Bienkowski, 1984a, b; Berg, 1986). It has been shown that 10-20% of all newly synthesized collagen is degraded by cells actively involved in its synthesis, and this degradation occurs prior to secretion.
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离子载体莫能菌素对培养软骨细胞 II 型胶原和蛋白聚糖合成和分泌的影响。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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影响因子：
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DOI：
10.1016/s0174-173x(83)80037-5
发表时间：
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期刊：
Collagen and related research
影响因子：
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作者：
A. Palotie
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影响因子：
2.9
作者：
Robert S. Bienkowski;Carol J. Engels
通讯作者：
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