Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species.
Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species.
复制标题
牙龈卟啉单胞菌菌毛及相关细菌种类的分布和免疫化学特异性。
DOI：
--
复制
发表时间：
1991
影响因子：
--
通讯作者：
Shigeyuki Hamada
中科院分区：
文献类型：
--
作者：
Tomohiko Ogawa;Tetsu Mukai;Kenji Yasuda;H. Shimauchi;Yoshihisa Toda;Shigeyuki Hamada
制备了牙龈卟啉单胞菌381菌毛特异性兔多克隆抗体（Poly-1）和鼠单克隆抗体（mAb）TO-11、TO-14和TO-M1。通过酶联免疫吸附试验（ELISA）和使用[125 I]Poly-1和[125 I] mAb的结合实验，筛选Poly-1和3种mAb与全细胞口腔和非口腔黑色色素细菌菌种的反应性。ELISA检测结果显示，Poly-1与11株牙龈卟啉单胞菌的全细胞均呈阳性反应。然而，11株牙龈卟啉单胞菌中有8株与mAb TO-11、TO-14和TO-M1反应。放射性标记的Poly-1和mAb TO-11与8种菌株的特异性结合证实了这些结果。从牙龈卟啉单胞菌381、BH 18/10、HW 24 D-1、6/26和OMZ 314中分离纯化的菌毛亚基蛋白（fimbrilin）经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析，其分子量为41 kDa。免疫印迹结果表明，TO-11和TO-14/TO-M1单抗识别牙龈卟啉单胞菌菌毛蛋白的表位不同。牙龈卟啉单胞菌菌株的全细胞和纯化的菌毛的免疫电子显微照片显示了与菌毛类似的表型特异性抗体结合。这些结果表明，11株牙龈卟啉单胞菌可以根据菌毛的免疫化学特异性分为至少2个独立的组。
Rabbit polyclonal antibody (Poly-1) and mouse monoclonal antibodies (mAbs) TO-11, TO-14 and TO-M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly-1 and the 3 mAbs were screened for their reactivity with whole cells oral and nonoral black-pigmented bacterial species by enzyme-linked immunosorbent assay (ELISA) and the binding experiment using [125I]Poly-1 and [125I]mAbs. ELISA revealed that Poly-1 definitely reacted with whole cells of all the 11 strains of P. gingivalis tested. However, 8 of 11 P. gingivalis strains reacted with mAbs TO-11, TO-14 and TO-M1. These results were confirmed by the specific binding of radiolabelled Poly-1 and mAb TO-11 to the 8 strains. The M(r) of the fimbrial subunit protein (fimbrilin) isolated and purified from P. gingivalis strains 381, BH18/10, HW24D-1, 6/26 and OMZ 314 was 41 kDa by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It was found by immunoblotting that mAbs TO-11 and TO-14/TO-M1 recognized different epitopes of fimbrial protein from P. gingivalis strains. Immunoelectron micrographs of whole cells and the purified fimbriae of P. gingivalis strains visualized similar serotype-specific antibody bindings to the fimbriae. These results indicate that 11 strains of P. gingivalis could be divided into at least 2 separate groups based on the immunochemical specificities of the fimbriae.
牙周病中牙龈拟杆菌特异性血清 IgG 和 IgA 亚类抗体。
影响因子：
4.6
作者：
Ogawa,T;Kusumoto,Y;Hamada,S;McGhee,JR;Kiyono,H
通讯作者：
Kiyono,H