A potentiometric formaldehyde biosensor based on immobilization of alcohol oxidase on acryloxysuccinimide-modified acrylic microspheres.
A potentiometric formaldehyde biosensor based on immobilization of alcohol oxidase on acryloxysuccinimide-modified acrylic microspheres.
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DOI：
10.3390/s101109963
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Heng LY
中科院分区：
文献类型：
--
作者：
Ling YP;Heng LY
开发了一种基于丙烯酸微球的新型醇氧化酶（AOX）酶基甲醛生物传感器。采用乳液形式的光聚合合成了疏水性聚（丙烯酸正丁酯-N-丙烯酰氧基-琥珀酰亚胺）[聚（nBA-NAS）]微球（一种酶固定基质）。 AOX-poly(nBA-NAS) 微球沉积在 pH 传感器上，该 pH 传感器由一层光固化和自增塑聚丙烯酸酯膜制成，并在 Ag/AgCl 丝网印刷电极 (SPE) 上涂有包埋的 pH 离子载体。固定 AOX 氧化甲醛会产生质子，可通过 pH 传感器测定。研究了缓冲液浓度、pH 值和不同量的固定基质对生物传感器分析性能的影响。甲醛生物传感器对甲醛表现出 0.3–316.2 mM 的动态线性响应范围和 59.41 ± 0.66 mV/decade 的灵敏度（R2 = 0.9776，n = 3）。生物传感器的检测下限为0.3 mM，重现性和重复性分别为3.16% RSD（相对标准偏差）和1.11% RSD (n = 3)。与厚膜固定方法相比，在电位甲醛生物传感器中使用丙烯酸微球提高了生物传感器在响应时间、线性响应范围和长期稳定性方面的性能。
A new alcohol oxidase (AOX) enzyme-based formaldehyde biosensor based on acrylic microspheres has been developed. Hydrophobic poly(n-butyl acrylate-N-acryloxy-succinimide) [poly(nBA-NAS)] microspheres, an enzyme immobilization matrix, was synthesized using photopolymerization in an emulsion form. AOX-poly(nBA-NAS) microspheres were deposited on a pH transducer made from a layer of photocured and self-plasticized polyacrylate membrane with an entrapped pH ionophore coated on a Ag/AgCl screen printed electrode (SPE). Oxidation of formaldehyde by the immobilized AOX resulted in the production of protons, which can be determined via the pH transducer. Effects of buffer concentrations, pH and different amount of immobilization matrix towards the biosensor’s analytical performance were investigated. The formaldehyde biosensor exhibited a dynamic linear response range to formaldehyde from 0.3–316.2 mM and a sensitivity of 59.41 ± 0.66 mV/decade (R2 = 0.9776, n = 3). The lower detection limit of the biosensor was 0.3 mM, while reproducibility and repeatability were 3.16% RSD (relative standard deviation) and 1.11% RSD, respectively (n = 3). The use of acrylic microspheres in the potentiometric formaldehyde biosensor improved the biosensor’s performance in terms of response time, linear response range and long term stability when compared with thick film immobilization methods.
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影响因子：
4.4
作者：
Bayramoglu, G;Yalçin, E;Arica, MY
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