Mouse Obox and Crxos modulate preimplantation transcriptional profiles revealing similarity between paralogous mouse and human homeobox genes.
Mouse Obox and Crxos modulate preimplantation transcriptional profiles revealing similarity between paralogous mouse and human homeobox genes.
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小鼠OBOX和CRXOS调节植入前转录曲线，揭示了寄生虫小鼠和人同源基因之间的相似性。
DOI：
10.1186/s13227-018-0091-4
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发表时间：
2018
期刊：
影响因子：
4.1
通讯作者：
Holland PWH
中科院分区：
文献类型：
--
作者：
Royall AH;Maeso I;Dunwell TL;Holland PWH
ETCHbox基因是真兽种特异性同源框基因，在着床前发育期间表达，此时正在进行第一细胞谱系决定。小鼠具有不寻常的ETCHbox基因库，其中有几个基因家族在进化中丢失，剩下的两个，Crxos和Obox，在序列和数量上有很大差异。每一个都经历了重复，得到一个双同源结构域Crxos基因座和一个超过60个Obox基因座的大集群。物种之间的基因含量差异提出了一个重要的问题，即进化如何能够容忍与关键发育事件有关的基因的丢失。我们发现Crxos内部复制发生在小鼠谱系中，而Obox复制是逐步的，产生具有不同序列和表达的亚组。三个Obox基因和Crxos转录本在原代小鼠胚胎细胞中的异位表达，然后进行转录组测序，从而可以研究它们的功能作用。我们发现不同的Obox亚群和Crxos，包括合子基因组激活和准备囊胚形成相关的基因的调制不同的转录组学的影响。与使用人类同源异型盒基因进行的类似实验的比较揭示了小鼠Crxos下游基因和人类ARGFX下游基因之间的惊人重叠。小鼠Crxos和人类ARGFX同源框基因是旁系同源而不是直系同源的，但它们已经进化到调节一组共同的基因。这表明，通过不同位点的共同选择，基因丢失的同时存在功能补偿。功能补偿非正交基因与不同序列是不寻常的，但可能表明潜在的分布式鲁棒性。补偿可能是由早期胚胎成功发育的强大进化压力驱动的。本文的在线版本（10.1186/s13227-018-0091-4）包含补充材料，可供授权用户使用。
ETCHbox genes are eutherian-specific homeobox genes expressed during preimplantation development at a time when the first cell lineage decisions are being made. The mouse has an unusual repertoire of ETCHbox genes with several gene families lost in evolution and the remaining two, Crxos and Obox, greatly divergent in sequence and number. Each has undergone duplication to give a double homeodomain Crxos locus and a large cluster of over 60 Obox loci. The gene content differences between species raise important questions about how evolution can tolerate loss of genes implicated in key developmental events. We find that Crxos internal duplication occurred in the mouse lineage, while Obox duplication was stepwise, generating subgroups with distinct sequence and expression. Ectopic expression of three Obox genes and a Crxos transcript in primary mouse embryonic cells followed by transcriptome sequencing allowed investigation into their functional roles. We find distinct transcriptomic influences for different Obox subgroups and Crxos, including modulation of genes related to zygotic genome activation and preparation for blastocyst formation. Comparison with similar experiments performed using human homeobox genes reveals striking overlap between genes downstream of mouse Crxos and genes downstream of human ARGFX. Mouse Crxos and human ARGFX homeobox genes are paralogous rather than orthologous, yet they have evolved to regulate a common set of genes. This suggests there was compensation of function alongside gene loss through co-option of a different locus. Functional compensation by non-orthologous genes with dissimilar sequences is unusual but may indicate underlying distributed robustness. Compensation may be driven by the strong evolutionary pressure for successful early embryo development. The online version of this article (10.1186/s13227-018-0091-4) contains supplementary material, which is available to authorized users.
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影响因子：
3.5
作者：
Alfano, G;Vitiello, C;Banfi, S
通讯作者：
Banfi, S
影响因子：
2.7
作者：
Reinholt, Brad M.;Bradley, Jennifer S.;Johnson, Sally E.
通讯作者：
Johnson, Sally E.
影响因子：
5.4
作者：
Holland PW;Booth HA;Bruford EA
通讯作者：
Bruford EA
影响因子：
4.6
作者：
Madissoon E;Jouhilahti EM;Vesterlund L;Töhönen V;Krjutškov K;Petropoulos S;Einarsdottir E;Linnarsson S;Lanner F;Månsson R;Hovatta O;Bürglin TR;Katayama S;Kere J
通讯作者：
Kere J
影响因子：
--
作者：
Holland, Peter W. H.
通讯作者：
Holland, Peter W. H.