The effect of inhibitors of nitric oxide biosynthesis and cyclic GMP formation on nerve‐evoked relaxation of human cavernosal smooth muscle
The effect of inhibitors of nitric oxide biosynthesis and cyclic GMP formation on nerve‐evoked relaxation of human cavernosal smooth muscle
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一氧化氮生物合成和环鸟苷酸形成抑制剂对人海绵体平滑肌神经诱发松弛的影响
DOI：
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发表时间：
1991
影响因子：
7.3
通讯作者：
M. Zar
中科院分区：
文献类型：
--
作者：
R. Pickard;P. H. Powell;M. Zar
1. 对人阴茎海绵体平滑肌离体标本的抑制性传递进行了研究。
2. 电场刺激（EFS；每串2 - 64个脉冲，脉冲持续时间0.8毫秒，10赫兹）可引起经胍乙啶（50μm）处理的标本舒张。EFS诱发的舒张对阿托品不敏感，但对河豚毒素敏感，表明其源于非肾上腺素能、非胆碱能（NANC）神经刺激。
3. EFS诱发的舒张被一氧化氮（NO）合酶抑制剂L - NG - 硝基精氨酸（L - NOARG；0.3 - 100μm）呈剂量依赖性减弱，但D - NG - 硝基精氨酸无此作用。L - NOARG对传递的抑制作用可被NO前体L - 精氨酸（100μm）拮抗，但D - 精氨酸无此作用。
4. 用亚甲蓝（10 - 50μm，一种已知的NO激活鸟苷酸环化酶的抑制剂）孵育，可引起与浓度相关的EFS诱发舒张的抑制。
5. 结论是，人海绵体平滑肌的NANC神经诱发舒张是由NO或一种类NO物质介导的。
1 The inhibitory transmission in isolated preparations of cavernosal smooth muscle from human penis has been studied. 2 Electrical field stimulation (EFS; 2–64 pulses/train, 0.8 ms pulse duration, 10 Hz) evoked relaxation of preparations treated with guanethidine (50 μm). The EFS‐evoked relaxations were atropine‐resistant and tetrodotoxin‐sensitive indicating their origin to be non‐adrenergic, non‐cholinergic (NANC) nerve stimulation. 3 EFS‐evoked relaxation was attenuated dose‐dependently by the nitric oxide (NO)‐synthase inhibitor, l‐NG‐nitro arginine (l‐NOARG; 0.3–100 μm) but not by d‐NG‐nitro arginine. The inhibitory effect of l‐NOARG on transmission was antagonized by l‐arginine (100 μm), a NO precursor, but not by d‐arginine. 4 Incubation with methylene blue (10–50 μm), a known inhibitor of guanylate cyclase activation by NO, caused a concentration‐related inhibition of EFS‐evoked relaxation. 5 It is concluded that NANC nerve‐evoked relaxation of human cavernosal smooth muscle is mediated by NO or a NO‐like substance.
DOI：
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发表时间：
1985-03
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
通讯作者：
W. Martin;G. Villani;D. Jothianandan;R. Furchgott
影响因子：
2.9
作者：
MARLETTA, MA;YOON, PS;WISHNOK, JS
通讯作者：
WISHNOK, JS
DOI：
10.1016/0006-291x(90)92168-y
发表时间：
1990-07-31
影响因子：
3.1
作者：
IGNARRO, LJ;BUSH, PA;RAJFER, J
通讯作者：
RAJFER, J