Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium.
Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium.
复制标题
过表达CmJAZ 1-like基因延迟菊花开花
DOI：
10.1038/s41438-021-00525-y
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发表时间：
2021-04-01
影响因子：
8.7
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Guan Y;Ding L;Jiang J;Shentu Y;Zhao W;Zhao K;Zhang X;Song A;Chen S;Chen F
菊花是世界四大切花植物之一，具有很高的观赏价值和文化内涵。由于大多数菊花品种在秋季开花，实现年产量是昂贵的。Tify家族的Jaz基因是茉莉酸(JA)信号通路的核心组成部分；除了在植物对防御反应中发挥关键作用外，它们还广泛参与调控植物的发育过程。在这里，我们鉴定了菊花品种‘金巴’中的Tify家族基因CmJAZ1-like。CmJAZ1-like定位于细胞核，在酵母中没有转录活性。组织表达谱分析表明，CmJAZ1类基因在根部和顶端活性最高。在菊花中过表达CmJAZ1-like和Jas结构域缺失会导致延迟开花。RNA-Seq分析揭示了一些与开花相关的差异表达基因，如开花整合子FT和SOC1的同源基因、与花分生组织鉴定有关的FUL同源基因、含AP2结构域的转录因子、MADS盒基因和自主途径相关基因。基于KEGG途径分析，差异转录基因在碳水化合物代谢途径和与脂肪酸相关的途径中得到丰富，这两个途径在植物开花过程中具有显著的作用。本研究初步验证了CmJAZ1类基因在菊花开花中的作用，可用于菊花开花时间调控的分子育种研究。
Chrysanthemum (Chrysanthemum morifolium) is one of the four major cut-flower plants worldwide and possesses both high ornamental value and cultural connotation. As most chrysanthemum varieties flower in autumn, it is costly to achieve annual production. JAZ genes in the TIFY family are core components of the jasmonic acid (JA) signaling pathway; in addition to playing a pivotal role in plant responses to defense, they are also widely implicated in regulating plant development processes. Here, we characterized the TIFY family gene CmJAZ1-like from the chrysanthemum cultivar ‘Jinba’. CmJAZ1-like localizes in the nucleus and has no transcriptional activity in yeast. Tissue expression pattern analysis indicated that CmJAZ1-like was most active in the root and shoot apex. Overexpressing CmJAZ1-like with Jas domain deletion in chrysanthemum resulted in late flowering. RNA-Seq analysis of the overexpression lines revealed some differentially expressed genes (DEGs) involved in flowering, such as the homologs of the flowering integrators FT and SOC1, an FUL homolog involved in flower meristem identity, AP2 domain-containing transcription factors, MADS box genes, and autonomous pathway-related genes. Based on KEGG pathway enrichment analysis, the differentially transcribed genes were enriched in carbohydrate metabolic and fatty acid-related pathways, which are notable for their role in flowering in plants. This study preliminarily verified the function of CmJAZ1-like in chrysanthemum flowering, and the results can be used in molecular breeding programs aimed at flowering time regulation of chrysanthemum.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1073/pnas.1811828115
发表时间：
2018-11-06
影响因子：
11.1
作者：
Guo Q;Yoshida Y;Major IT;Wang K;Sugimoto K;Kapali G;Havko NE;Benning C;Howe GA
通讯作者：
Howe GA
影响因子：
16.6
作者：
Cai, Qiang;Yuan, Zheng;Zhang, Dabing
通讯作者：
Zhang, Dabing
影响因子：
5.6
作者：
Li Q;Shao J;Tang S;Shen Q;Wang T;Chen W;Hong Y
通讯作者：
Hong Y
影响因子：
5.3
作者：
Chen, Xilong;Qi, Siyan;Xing, Libo
通讯作者：
Xing, Libo