Cryo-Electron Microscopy Reveals That Sperm Modification Coincides with Female Fertility in the Mosquito Aedes aegypti
Cryo-Electron Microscopy Reveals That Sperm Modification Coincides with Female Fertility in the Mosquito Aedes aegypti
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冷冻电子显微镜显示，埃及伊蚊的精子修饰与雌性生育能力一致
DOI：
10.1038/s41598-019-54920-6
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发表时间：
2019
影响因子：
4.6
通讯作者：
Kourkoutis, Lena F.
中科院分区：
文献类型：
--
作者：
Noble, Jade M.;Degner, Ethan C.;Harrington, Laura C.;Kourkoutis, Lena F.
控制蚊子繁殖是减少蚊子数量及其携带疾病负担的一种有前途的方法。制定此类策略需要全面了解生殖过程，但人们对精子如何在雌性蚊子体内加工和准备受精的情况知之甚少。通过首次使用冷冻电子显微镜研究埃及伊蚊的精子，我们发现精子在储存在雌性体内后 24 小时内就会脱落整个外层，即糖萼。活力测定表明，当雌性精子储存器官中的糖萼脱落时，精子就会从休眠期过渡到快速运动——这是精子到达卵子的关键先决条件。我们还表明，随着精子变得活跃，雌性逐渐变得具有生育能力，并且在雌性达到生育高峰后，产卵行为急剧增加。这些实验共同证明了伊蚊的几个繁殖事件的时间线惊人的巧合。埃及伊蚊，表明精子修饰与女性生殖能力之间存在直接关系。
Manipulating mosquito reproduction is a promising approach to reducing mosquito populations and the burden of diseases they carry. A thorough understanding of reproductive processes is necessary to develop such strategies, but little is known about how sperm are processed and prepared for fertilization within female mosquitoes. By employing cryo-electron microscopy for the first time to study sperm of the mosquitoAedes aegypti, we reveal that sperm shed their entire outer coat, the glycocalyx, within 24 hours of being stored in the female. Motility assays demonstrate that as their glycocalyx is shed in the female’s sperm storage organs, sperm transition from a period of dormancy to rapid motility—a critical prerequisite for sperm to reach the egg. We also show that females gradually become fertile as sperm become motile, and that oviposition behavior increases sharply after females reach peak fertility. Together, these experiments demonstrate a striking coincidence of the timelines of several reproductive events inAe. aegypti, suggesting a direct relationship between sperm modification and female reproductive capacity.
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影响因子：
3.8
作者：
Carvalho DO;McKemey AR;Garziera L;Lacroix R;Donnelly CA;Alphey L;Malavasi A;Capurro ML
通讯作者：
Capurro ML
DOI：
--
发表时间：
1953
期刊：
影响因子：
--
作者：
R. O. Hayes
通讯作者：
R. O. Hayes
储存的精子与射精的精子的不同之处在于与蜜蜂能量代谢相关的蛋白质组改变
影响因子：
4.9
作者：
Veronica F. Poland;Holger Eubel;M. King;Cory Solheim;A. Harvey Millar;B. Baer
通讯作者：
B. Baer
虎库蚊精子发生中核膜减少的过程
影响因子：
2.5
作者：
M. Ndiaye;X. Mattei
通讯作者：
X. Mattei
影响因子：
2.2
作者：
HISS, EA;FUCHS, MS
通讯作者：
FUCHS, MS