Beef, Casein, and Soy Proteins Differentially Affect Lipid Metabolism, Triglycerides Accumulation and Gut Microbiota of High-Fat Diet-Fed C57BL/6J Mice.
Beef, Casein, and Soy Proteins Differentially Affect Lipid Metabolism, Triglycerides Accumulation and Gut Microbiota of High-Fat Diet-Fed C57BL/6J Mice.
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牛肉、酪蛋白和大豆蛋白对高脂饮食喂养的 C57BL/6J 小鼠的脂质代谢、甘油三酯积累和肠道微生物群有不同影响
DOI：
10.3389/fmicb.2018.02200
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发表时间：
2018
影响因子：
5.2
通讯作者：
Li C
中科院分区：
文献类型：
--
作者：
Ijaz MU;Ahmed MI;Zou X;Hussain M;Zhang M;Zhao F;Xu X;Zhou G;Li C
摄入推荐水平的膳食蛋白质被认为是促进饱腹感和体重管理的潜在策略，但来自不同饮食来源的蛋白质如何影响肥胖发展、脂肪代谢和肠道微生物区系尚不清楚。本研究以低脂饲料(12%KCAL)和高脂饲料(HFD，60%KCAL)饲喂C57BL/6J小鼠12周，研究了牛肉、酪蛋白和大豆蛋白对小鼠结肠脂代谢、甘油三酯积累和微生物多样性的影响。饲喂牛肉蛋白HFD(HFB)的小鼠体重和肝脏重量显著增加，而大豆蛋白HFD(HFS)组小鼠的累积能量摄入量减少。高脂饲料喂养的小鼠表现出糖代谢受损和胰岛素抵抗的迹象，同时血清中甘油三酯、低密度脂蛋白-胆固醇、总胆固醇、IL-1β、肿瘤坏死因子-α、IL-6和瘦素的浓度显著升高。HFB还增加了脂肪在肝脏中的积累，增加了脂肪生成和肝脏胆固醇代谢重要基因的活性。16S rRNA基因测序表明，HFD无论是什么蛋白质，都显著增加了结肠微生物区系中的Firmicuts和类杆菌的比例。然而，与不依赖蛋白质的LFD相比，HFB不仅降低了阿克曼氏菌的丰度，而且与HFS和HFC相比，还降低了丁酸产生菌如厌氧主干菌、丁酸菌和乳杆菌的丰度(P<；0.05)。总之，食用HFB不仅会影响肠道微生物区系组成，还会增加与代谢综合征相关的问题，如血脂异常、高胆固醇血症和甘油三酯在肝脏中的堆积，从而导致全身炎症及其相关的共病，如葡萄糖代谢受损和胰岛素抵抗。
Consumption of dietary protein at recommended levels is considered a potential strategy to promote satiety and weight management, but how protein from different dietary sources effect the obesity development, lipid metabolism, and gut microbiota is not known. This study focused on the effects of beef, casein, and soy protein diet on lipid metabolism, triglycerides accumulation, and microbial diversity in colon of C57BL/6J mice, which were given either low-fat diets (LFD, 12% Kcal) or high-fat diets (HFD, 60% Kcal) for 12 weeks. Body and liver weight increased significantly in mice fed a beef protein HFD (HFB), whereas reduced cumulative energy intake was seen in a soy protein HFD (HFS) group. HFB-fed mice showed signs of impaired glucose metabolism and insulin resistance along with a significant elevation in the concentration of triglycerides, LDL-cholesterol, total cholesterol, IL1β, TNF-α, IL-6, and leptin in serum. HFB also enhanced lipid accumulation in liver with increased activity of genes important for lipogenesis and hepatic cholesterol metabolism. A 16S rRNA gene sequencing indicated that HFD, regardless of proteins, significantly enhanced the ratio of Firmicutes to Bacteroidetes in colonic microbiota. However, HFB not only reduced the abundance of Akkermansia, compared with LFD independent of proteins, but also decreased the abundance of butyrate-producing bacteria such as Anaerotruncus, Butyricicoccus, and Lactobacillus (P < 0.05) compared with HFS and HFC. In conclusion, consumption of HFB does not only affect the gut microbiota composition but also increases the problems related to metabolic syndromes like dyslipidemia, hypercholesterolemia, and triglycerides accumulation in liver, which lead to systemic inflammation and its associated comorbidities, for example, impaired glucose metabolism and insulin resistance.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
29
作者：
Min HK;Kapoor A;Fuchs M;Mirshahi F;Zhou H;Maher J;Kellum J;Warnick R;Contos MJ;Sanyal AJ
通讯作者：
Sanyal AJ
DOI：
10.1038/ismej.2012.153
发表时间：
2013-04
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
1.6
作者：
Kitano, Yuki;Murazumi, Koki;Hirata, Takashi
通讯作者：
Hirata, Takashi