Rapid decline of adaptation of Pseudomonas fluorescens to soil biotic environment.
Rapid decline of adaptation of Pseudomonas fluorescens to soil biotic environment.
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DOI：
10.1098/rsbl.2021.0593
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发表时间：
2022-03
期刊：
影响因子：
3.3
通讯作者：
Buckling A
中科院分区：
文献类型：
--
作者：
Gómez P;Hall AR;Paterson S;Buckling A
微生物之间的相互作用既可以限制也可以增强它们对环境的适应。然而，迄今为止，大多数研究都采用了简化的微生物群落和环境条件。我们确定了商业盆栽堆肥微生物群落的存在如何影响土壤细菌荧光假单胞菌SBW25在盆栽堆肥中的适应。从种群中分离的荧光假单胞菌克隆在群落存在和不存在的情况下，在没有群落的情况下进行测量时，显示出类似的适合度增加。这表明群落的存在并没有限制适应。相比之下，在群落存在的情况下衡量的适合度对于社区进化的种群来说是增加的，但对于在没有群落的情况下进化的种群则降低到祖先状态以下。这表明，一些(但不是全部)对非生物环境有利的突变在社区存在时代价高昂，前者在社区存在时被选为反对突变。全基因组测序支持这种解释：大多数支持适应性变化的突变是克隆特有的，这表明有多种基因路径可以适应。这种极端的突变效应在可比的体外研究中没有观察到，这表明在将简化的体外系统的结果外推到自然环境中时需要谨慎。
Interactions between microbes can both constrain and enhance their adaptation to the environment. However, most studies to date have employed simplified microbial communities and environmental conditions. We determined how the presence of a commercial potting compost microbial community affected adaptation of the soil bacterium Pseudomonas fluorescens SBW25 in potting compost. Pseudomonas fluorescens clones isolated from populations evolved in both the presence and absence of the community showed similar fitness increases when measured in the absence of the community. This suggests the presence of the community did not constrain adaptation. By contrast, fitness measured in the presence of the community increased for community-evolved populations, but decreased below the ancestral state for populations evolved in the absence of the community. This suggests some, but not all, mutations that were beneficial with respect to the abiotic environment were costly in the presence of the community, with the former selected against in the presence of the community. Whole-genome sequencing supports this interpretation: most mutations underpinning fitness changes were clone-specific, suggesting multiple genetic pathways to adaptation. Such extreme mutational effects have not been observed in comparable in vitro studies, suggesting that caution is needed when extrapolating results from simplified in vitro systems to natural contexts.
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DOI：
10.1098/rspb.2014.2297
发表时间：
2015-01-07
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
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通讯作者：
Buckling A
影响因子：
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影响因子：
2.1
作者：
van Houte S;Padfield D;Gómez P;Luján AM;Brockhurst MA;Paterson S;Buckling A
通讯作者：
Buckling A