Complete genome analysis of a novel norovirus GII.4 variant identified in China
Complete genome analysis of a novel norovirus GII.4 variant identified in China
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中国发现的新型诺如病毒 GII.4 变种的完整基因组分析
DOI：
10.1007/s11262-013-0945-8
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发表时间：
2013-07
期刊：
影响因子：
1.6
通讯作者：
Guo, Weipeng
中科院分区：
文献类型：
--
作者：
Kou, Xiaoxia;Cai, Weicheng;Zhang, Jumei;Guo, Weipeng
本研究对在广州发现的一株诺如病毒新株GZ 2010-L 87的全基因组序列进行了系统发育分析。GZ 2010-L 87菌株的RNA基因组由7，559个核苷酸组成。基于开放阅读框（ORF）2的系统发育分析显示，该菌株属于GII. 4基因型，形成了新的GII. 4 -2009簇，该簇自2009年以来也在亚洲和美国被鉴定。此外，对GZ 2010-L 87和其他代表菌株的全基因组和不同开放阅读框序列的系统发育分析表明，新菌株没有发生重组。与31个完全测序的诺如病毒GII.4-2009基因组的共有序列进行比较分析，发现86个核苷酸错配（ORF 1中56个，ORF 2中16个，ORF 3中14个），导致19个氨基酸变化（ORF 1中9个，ORF 2中3个，ORF 3中7个）。此外，在诺如病毒GII. 4 -2009衣壳蛋白上发现了12个可变位点，且大多数位于P2结构域。同时，基于与其他GII.4簇的比较，显示了14个位点特异于新簇。总之，在中国首次发现的新GII.4-2009变异体GZ 2010-L 87的基因组被广泛表征为具有大量遗传多样性的诺如病毒。从新变异株中获得的基因组信息不仅可以作为中国诺如病毒全长序列的标准，而且可以作为未来进化研究的参考数据。
The complete genome sequence of a novel norovirus strain GZ2010-L87 identified in Guangzhou was analyzed phylogenetically in this study. The RNA genome of the GZ2010-L87 strain is composed of 7,559 nucleotides. The phylogenetic analysis based on open reading frame (ORF) 2 revealed that the strain belongs to the GII.4 genotype, forming the new cluster GII.4-2009 which was also identified in Asia and the USA since 2009. Furthermore, phylogenetic analyses of the full genome and the different open reading frame sequences of GZ2010-L87 and other representative strains suggested that the novel strain did not undergo recombination. Comparative analysis with the consensus sequence of 31 completely sequenced norovirus GII.4-2009 genomes showed 86 mismatched nucleotides (56 in ORF1, 16 in ORF2, and 14 in ORF3), resulting in 19 amino acid changes (9 in ORF1, 3 in ORF2, and 7 in ORF3). Furthermore, 12 variable sites were found on the capsid protein of norovirus GII.4-2009, and most were located at the P2 domain. Meanwhile, based on comparison with other GII.4 clusters, 14 sites were shown specific to the novel cluster. In summary, the genome of the new GII.4-2009 variant GZ2010-L87, which was first identified in China, was extensively characterized with a large panel of genetically diverse noroviruses. The genomic information obtained from the novel variant can be used not only as a full-length norovirus sequence standard in China but also as reference data for future evolution research.
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影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s0140-6736(15)00108-7
发表时间：
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期刊：
The Lancet
影响因子：
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作者：
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通讯作者：
B. Bynum;H. Bynum
影响因子：
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作者：
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通讯作者：
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