Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins
Marked differences in the antigenic structure of human respiratory syncytial virus F and G glycoproteins
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人呼吸道合胞病毒F和G糖蛋白抗原结构的显着差异
DOI：
10.1128/jvi.63.2.925-932.1989
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发表时间：
1989
影响因子：
5.4
通讯作者：
J. Melero
中科院分区：
文献类型：
--
作者：
B. García;C. Palomo;'. C. Penas;T. Delgado;P. Perez;J. Melero
针对人呼吸道合胞病毒糖蛋白的单克隆抗体用于竞争性酶联免疫吸附测定表位的拓扑定位。而F糖蛋白的表位可归因于五个不重叠的抗原位点，抗g抗体识别独特的表位，其中许多竞争谱广泛重叠。用中和（47F）抗f抗体选择的变异病毒仅与47F和49F抗体失去结合，这两个抗体位于相同的抗原区。相比之下，选择抗G抗体的病毒失去了与大多数抗G抗体结合的能力，并且它们的G蛋白不能被抗病毒抗血清识别，这表明G分子的抗原结构发生了重大变化。最后，我们发现不同病毒分离株的G蛋白存在很大的抗原差异。这种情况甚至发生在同一亚型的病毒中，菌株之间的氨基酸序列差异有限。这些数据表明呼吸道合胞病毒G糖蛋白和F糖蛋白的抗原组织存在显著差异；我们根据糖蛋白的化学结构来讨论这些差异。
Monoclonal antibodies directed against the glycoproteins of human respiratory syncytial virus were used in competitive enzyme-linked immunosorbent assays for topological mapping of epitopes. Whereas epitopes of the F glycoprotein could be ascribed to five nonoverlapping antigenic sites, anti-G antibodies recognized unique epitopes, many of whose competition profiles overlapped extensively. Variant viruses selected with a neutralizing (47F) anti-F antibody lost the binding for only 47F and 49F antibodies, which mapped in the same antigenic area. In contrast, viruses selected with an anti-G antibody lost the capacity to bind most of the anti-G antibodies, and their G protein was not recognized by an anti-virus antiserum, indicating major changes in the antigenic structure of the G molecule. Finally, we found great antigenic variation of the G protein among viral isolates. This occurred even within viruses of the same subtype with only limited divergence of amino acid sequence between strains. All of these data indicate marked differences in the antigenic organization of the G and F glycoproteins of respiratory syncytial virus; we discuss these differences in terms of the chemical structure of the glycoproteins.
DOI：
10.1073/pnas.82.12.4075
发表时间：
1985-06
影响因子：
11.1
作者：
G. Wertz;P. Collins;Yung;Huang;C. Gruber;S. Levine;L. Ball
通讯作者：
G. Wertz;P. Collins;Yung;Huang;C. Gruber;S. Levine;L. Ball
DOI：
10.1073/pnas.81.6.1779
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SKEHEL, JJ;STEVENS, DJ;WILEY, DC
通讯作者：
WILEY, DC