Characterization of the ERP gene family in Arabidopsis thaliana

Characterization of the ERP gene family in Arabidopsis thaliana
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拟南芥 ERP 基因家族的特征

DOI:
10.1080/15592324.2021.1913301
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发表时间:
2021-04
影响因子:
2.9
通讯作者:
Gao Xin-Qi
Gao Xin-Qi
中科院分区:
生物学4区
文献类型:
--
作者:
Yu Cai Yu;Zhang Huan Kai;Wang Ning;Sun Jing;Dong Yu Xiu;Zhang Xian Sheng;Gao Xin-Qi

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摘要植物基因组编码许多缺乏可识别结构域或基序的模糊特征蛋白(POFs)。然而,即使在拟南芥中,POFs的功能信息也很少,因为POFs的功能注释需要生化,生理和遗传分析。在这里,我们确定了一个小的基因家族,内质网定位POF(ERP)家族,在拟南芥。系统发育分析表明,ERP家族成员的数量在植物界是保守的,表明ERP家族在植物进化过程中受到了强大的选择压力。除了N-末端信号肽外,在预测的ERP蛋白中没有识别出可识别的结构域。ERPs在拟南芥的发育过程中广泛表达,并表现为内质网定位。ERP 1是一种肌醇-1,4,5-三磷酸5-磷酸酶(5 PTase),但在花粉萌发过程中不能替代At 5 PTase 12,表明ERP 1在肌醇-1,4,5-三磷酸[Ins(1,4,5)P3]代谢中不具有与At 5 PTase 12相似的功能。ERP家族蛋白在拟南芥发育中的功能有待进一步研究。
ABSTRACT Plant genomes encode numerous proteins with obscure features (POFs) that lack recognized domains or motifs. However, there is little functional information for POFs even in Arabidopsis because biochemical, physiological, and genetic assay are required for the functional annotations of POFs. Here, we identified a small gene family, the endoplasmic reticulum-localized POF (ERP) family, in Arabidopsis. Phylogenetic analysis revealed that the number of ERP family members was conserved in the plant kingdom, suggesting strong selective pressure was imposed on ERP family during plant evolution. No recognizable domains were identified in the predicted ERP proteins, except for the N-terminal signal peptide. ERPs were found to be widely expressed during Arabidopsis development and showed endoplasmic reticulum localization. It was reported that ERP1 is an inositol-1,4,5-trisphosphate 5-phosphatase (5PTase), but ERP1 could not substitute for At5PTase12 in precocious pollen germination, indicating that ERP1 did not have the similar functions as At5PTase12 in inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] metabolism. Further studies are needed to dissect the functions of ERP family proteins in Arabidopsis development.
异常花粉管 GUIDANCE1 是拟南芥花粉管珠孔 Gu 所必需的,它是酵母中糖基磷酸肌醇 10 和人类中磷脂酰肌醇聚糖锚生物合成 B 的内质网定位甘露糖基转移酶同源物
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