QQS orphan gene and its interactor NF-YC4 reduce susceptibility to pathogens and pests.
QQS orphan gene and its interactor NF-YC4 reduce susceptibility to pathogens and pests.
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DOI：
10.1111/pbi.12961
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发表时间：
2019-01
影响因子：
13.8
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Qi M;Zheng W;Zhao X;Hohenstein JD;Kandel Y;O'Conner S;Wang Y;Du C;Nettleton D;MacIntosh GC;Tylka GL;Wurtele ES;Whitham SA;Li L
在不牺牲生产力的情况下提高作物的营养质量和抗病能力，是开发对农民有价值的品种以及同时改善粮食安全和可持续性的关键问题。拟南芥物种特有的AtQQS(Qua-Quine淀粉)孤儿基因或其相互作用因子NF-YC4(核因子Y，C4亚基)的表达被证明在不影响农艺物种的生长和产量的情况下增加了叶片/种子蛋白的水平。在这里，我们证明了AtQQS和NF-YC4在拟南芥和大豆中的过表达增强了对病毒、细菌、真菌、蚜虫和大豆胞囊线虫的抗性/降低了对病毒、细菌、真菌、蚜虫和大豆胞囊线虫的敏感性。以一系列拟南芥淀粉代谢突变体为材料，探讨了qqs的表达与碳、氮分配和防御的关系。QQS介导的增强的基础防御与植物蛋白质/碳水化合物组成的变化无关。我们证明了AtQQS或NF-YC4在拟南芥和大豆中的过表达降低了这些植物对病原体/害虫的敏感性。过量表达NF-YC4的转基因大豆在保持健康生长的同时，种子的蛋白质含量也有所增加。下拉研究表明，QQS与人的NF-YC和拟南芥的NF-YC4相互作用，并在NF-YC-组蛋白结合域附近发现了两个QQS结合部位。推测了一种新的QQS与Nf-YC相互作用模型。我们的发现说明了QQS和NF-YC4在增加蛋白质和改善作物防御性状方面的潜力，克服了正常的生长和防御之间的权衡。
Enhancing the nutritional quality and disease resistance of crops without sacrificing productivity is a key issue for developing varieties that are valuable to farmers and for simultaneously improving food security and sustainability. Expression of the Arabidopsis thaliana species‐specific AtQQS (Qua‐Quine Starch) orphan gene or its interactor, NF‐YC4 (Nuclear Factor Y, subunit C4), has been shown to increase levels of leaf/seed protein without affecting the growth and yield of agronomic species. Here, we demonstrate that overexpression of AtQQS and NF‐YC4 in Arabidopsis and soybean enhances resistance/reduces susceptibility to viruses, bacteria, fungi, aphids and soybean cyst nematodes. A series of Arabidopsis mutants in starch metabolism were used to explore the relationships between QQS expression, carbon and nitrogen partitioning, and defense. The enhanced basal defenses mediated by QQS were independent of changes in protein/carbohydrate composition of the plants. We demonstrate that either AtQQS or NF‐YC4 overexpression in Arabidopsis and in soybean reduces susceptibility of these plants to pathogens/pests. Transgenic soybean lines overexpressing NF‐YC4 produce seeds with increased protein while maintaining healthy growth. Pull‐down studies reveal that QQS interacts with human NF‐YC, as well as with Arabidopsis NF‐YC4, and indicate two QQS binding sites near the NF‐YC‐histone‐binding domain. A new model for QQS interaction with NF‐YC is speculated. Our findings illustrate the potential of QQS and NF‐YC4 to increase protein and improve defensive traits in crops, overcoming the normal growth‐defense trade‐offs.
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DOI：
10.1094/mpmi-21-10-1285
发表时间：
2008-10
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Genger RK;Jurkowski GI;McDowell JM;Lu H;Jung HW;Greenberg JT;Bent AF
通讯作者：
Bent AF
影响因子：
11.6
作者：
Clarke, JD;Liu, YD;Dong, XN
通讯作者：
Dong, XN
影响因子：
11.6
作者：
Bowling, SA;Clarke, JD;Dong, XN
通讯作者：
Dong, XN
影响因子：
4.9
作者：
Huitema, E;Vleeshouwers, VGAA;Kamoun, S
通讯作者：
Kamoun, S
影响因子：
3.5
作者：
Badel, JL;Shimizu, R;Collmer, A
通讯作者：
Collmer, A