Corrole-Substituted Fluorescent Heme Proteins.
Corrole-Substituted Fluorescent Heme Proteins.
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DOI：
10.1021/acs.inorgchem.0c03599
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发表时间：
2021-02-15
影响因子：
4.6
通讯作者：
Marletta MA
中科院分区：
文献类型：
--
作者：
Lemon CM;Marletta MA
虽然荧光蛋白已被用于各种生物学应用，但它们具有几个光学限制，即微弱的红光和NIR发射以及异常宽（> 200 nm）的发射谱。荧光蛋白的生物物理性质可以通过将具有所需性质的新型辅因子掺入稳定的蛋白质支架中来增强。为此，荧光磷咔咯，结构上类似于天然血红素辅因子被纳入两个非常稳定的血红素蛋白质：H-NOX从Caldanaerobacterium submergyeus和HasA从铜绿假单胞菌。这些黄橙色发射蛋白质共轭物的稳态和时间分辨光谱检查。HasA缀合物表现出增强的荧光，而来自H-NOX缀合物的发射相对于游离咔咯被淬灭。尽管低荧光量子产率，这些腐蚀取代的蛋白质表现出更强的荧光在一个较窄的光谱轮廓比传统的荧光蛋白，在相同的光谱窗口发射。这项研究表明，荧光咔咯复合物很容易纳入血红素蛋白，并提供了一个新的荧光蛋白的开发进展。
Although fluorescent proteins have been utilized for a variety of biological applications, they have several optical limitations, namely weak red and NIR emission and exceptionally broad (> 200 nm) emission profiles. The photophysical properties of fluorescent proteins can be enhanced through the incorporation of novel cofactors with the desired properties into a stable protein scaffold. To this end, a fluorescent phosphorus corrole that is structurally similar to the native heme cofactor is incorporated into two exceptionally stable heme proteins: H-NOX from Caldanaerobacter subterraneus and HasA from Pseudomonas aeruginosa. These yellow-orange emitting protein conjugates are examined by steady-state and time-resolved optical spectroscopy. The HasA conjugate exhibits enhanced fluorescence, whereas emission from the H-NOX conjugate is quenched relative to the free corrole. Despite the low fluorescence quantum yields, these corrole-substituted proteins exhibit more intense fluorescence in a narrower spectral profile than traditional fluorescent proteins that emit in the same spectral window. This study demonstrates that fluorescent corrole complexes are readily incorporated into heme proteins and provides an inroad for the development of novel fluorescent proteins.
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影响因子：
2.8
作者：
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通讯作者：
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通讯作者：
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作者：
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