Based on clinical Ki-67 expression and serum infiltrating lymphocytes related nomogram for predicting the diagnosis of glioma-grading.
Based on clinical Ki-67 expression and serum infiltrating lymphocytes related nomogram for predicting the diagnosis of glioma-grading.
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DOI：
10.3389/fonc.2022.696037
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发表时间：
2022
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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作者：
令人信服的证据表明，外周血淋巴细胞升高与各种癌症的良好预后有关。然而，血清淋巴细胞与胶质瘤之间的关系是矛盾的。本研究通过Ki-67表达和血清淋巴细胞建立了一种预测胶质瘤分级诊断的nomogram。我们对239例诊断为LGG和178例诊断为HGG的患者进行了回顾性分析。免疫组化检测Ki-67表达。通过多因素logistic回归分析，建立了一个nomogram，并用于识别与HGG相关的最相关因素。采用一致性指数（C-index）、决策曲线分析（DCA）和校准曲线对模型进行验证。IDH1/2突变和1p19q共缺失较多的LGG患者数量多于HGG患者。多因素logistic分析发现Ki-67表达、血清淋巴细胞计数和血清白蛋白（ALU）是与HGG相关的独立危险因素，这些因素被纳入训练队列的nomogram。在验证队列中，nomogram校正性好，一致性高（C-index = 0.794）。Spearman相关分析显示，HGG与血清淋巴细胞计数（r = - 0.238, P <0.001）、ALU （r = - 0.232, P <0.001）和Ki-67表达（r = 0.457, P <0.001）有显著相关性。Ki-67表达与血清淋巴细胞计数呈负相关（r = - 0.244, P <0.05）。与HGG患者相比，LGG患者Ki-67表达水平较低，血清淋巴细胞水平较高，且这两个变量的组合在HGG患者中明显较高。所构建的图能够预测胶质瘤级别的诊断。HGG患者血清淋巴细胞计数下降，Ki-67表达升高，说明免疫功能减弱，肿瘤侵袭性增强。
Compelling evidence indicates that elevated peripheral serum lymphocytes are associated with a favorable prognosis in various cancers. However, the association between serum lymphocytes and glioma is contradictory. In this study, a nomogram was established to predict the diagnosis of glioma-grading through Ki-67 expression and serum lymphocytes. We performed a retrospective analysis of 239 patients diagnosed with LGG and 178 patients with HGG. Immunohistochemistry was used to determine the Ki-67 expression. Following multivariate logistic regression analysis, a nomogram was established and used to identify the most related factors associated with HGG. The consistency index (C-index), decision curve analysis (DCA), and a calibration curve were used to validate the model. The number of LGG patients with more IDH1/2 mutations and 1p19q co-deletion was greater than that of HGG patients. The multivariate logistic analysis identified Ki-67 expression, serum lymphocyte count, and serum albumin (ALU) as independent risk factors associated with HGG, and these factors were included in a nomogram in the training cohort. In the validation cohort, the nomogram demonstrated good calibration and high consistency (C-index = 0.794). The Spearman correlation analysis revealed a significant association between HGG and serum lymphocyte count (r = −0.238, P <0.001), ALU (r = −0.232, P <0.001), and Ki-67 expression (r = 0.457, P <0.001). Furthermore, the Ki-67 expression was negatively correlated with the serum lymphocyte count (r = −0.244, P <0.05). LGG patients had lower Ki-67 expression and higher serum lymphocytes compared with HGG patients, and a combination of these two variables was significantly higher in HGG patients. The constructed nomogram is capable of predicting the diagnosis of glioma-grade. A decrease in the level of serum lymphocyte count and increased Ki-67 expression in HGG patients indicate that their immunological function is diminished and the tumor is more aggressive.
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DOI：
10.1016/j.jtcvs.2019.04.084
发表时间：
2019-09
期刊：
The Journal of thoracic and cardiovascular surgery
影响因子：
--
作者：
Mitchell KG;Parra ER;Nelson DB;Zhang J;Wistuba II;Fujimoto J;Roth JA;Antonoff MB;MD Anderson Lung Cancer Immune Microenvironment Working Group
通讯作者：
MD Anderson Lung Cancer Immune Microenvironment Working Group
影响因子：
50.5
作者：
Guarneri, V;Dieci, M., V;Conte, P. F.
通讯作者：
Conte, P. F.
影响因子：
7.2
作者：
Marinari, Eliana;Allard, Mathilde;Dietrich, Pierre-Yves
通讯作者：
Dietrich, Pierre-Yves
影响因子：
5.3
作者：
Ginhoux F;Lim S;Hoeffel G;Low D;Huber T
通讯作者：
Huber T
影响因子：
3.5
作者：
Patil, Vikas;Mahalingam, Kulandaivelu
通讯作者：
Mahalingam, Kulandaivelu