Structural evaluation of the peritubular sheath of rat's testes after administration of ribavirin: A possible impact on the testicular function.
Structural evaluation of the peritubular sheath of rat's testes after administration of ribavirin: A possible impact on the testicular function.
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DOI：
10.1177/0394632017726261
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发表时间：
2017-09
影响因子：
3.5
通讯作者：
Elbastawisy YM
中科院分区：
文献类型：
--
作者：
Almasry SM;Hassan ZA;Elsaed WM;Elbastawisy YM
研究了利巴韦林对精小管管周鞘（PS）结构及睾丸功能的影响。我们发现，与对照组相比，剂量为4 mg/kg/天的利巴韦林4周显著降低睾酮水平（6.3±0.2;P < 0.001）和生精评分计数（3.8±0.2;P < 0.001）。PS厚度（17.8±1.13）和管腔周长（1024.7±67）均显著高于对照组（10.7±0.70,P < 0.001和808±25，P = 0.004）。与对照组（708.4±40,P < 0.001）和2358.8±169,P < 0.001)相比，生发上皮长度（411.8±39）和管外径（1661.8±115）显著减少。基底膜（BMs）增厚，胶原大量沉积。肌样细胞结构改变，细胞外基质因I型和IV型胶原积累过多而紊乱。睾丸损伤经组织学证实。生殖细胞中检测到凋亡的证据。与对照组（24,788,409±1,900,140）相比，精管中Casp-3表达的综合密度（38,121,743±1,763,420;P < 0.001）显著增加。由此可见，利巴韦林给药4周后可引起睾丸功能和结构的改变，细胞凋亡增加。这种破坏作用可以通过破坏小管周围鞘而被说服。
Effects of ribavirin on the structure of peritubular sheath (PS) of seminiferous tubules and on testicular functions were studied. We found that ribavirin at a dose of 4 mg/kg/day for 4 weeks produced a significant reduction in testosterone level (6.3 ± 0.2; P < 0.001) and in spermatogenic score count (3.8 ± 0.2; P < 0.001) compared to control values. The thickness of PS (17.8 ± 1.13) and tubular lumen perimeter (1024.7 ± 67) was significantly increased compared to controls (10.7 ± 0.70; P < 0.001 and 808 ± 25; P = 0.004, respectively). The length of germinal epithelium (411.8 ± 39) and tubular external diameters (1661.8 ± 115) was significantly reduced compared to control values (708.4 ± 40; P < 0.001 and 2358.8 ± 169; P < 0.001, respectively). The basement membranes (BMs) were thickened with great deposition of collagen. Myoid cells showed altered structure and extracellular matrix revealed disorganization by excessive collagen I and IV accumulation. Testicular damage was established histologically. Evidence of apoptosis was detected in germ cells. There was a significant increase in integrated density of Casp-3 expression (38,121,743 ± 1,763,420; P < 0.001) in seminiferous tubules compared to control (24,788,409 ± 1,900,140). It is concluded that ribavirin can cause alterations of the testicular function and structure with increased apoptosis in the tissues after 4 weeks of administration. The damaging effect could be persuaded by destruction of the peritubular sheath.
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影响因子：
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