A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T.
A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T.
复制标题
DOI：
10.1111/j.1462-2920.2010.02336.x
复制
发表时间：
2011-08
影响因子：
5.1
通讯作者：
Kunte HJ
中科院分区：
文献类型：
--
作者：
Schwibbert K;Marin-Sanguino A;Bagyan I;Heidrich G;Lentzen G;Seitz H;Rampp M;Schuster SC;Klenk HP;Pfeiffer F;Oesterhelt D;Kunte HJ
嗜盐嗜盐变形盐单胞菌DSM2581T通过合成和积累相容的溶质胞外生碱，在高盐度条件下生长旺盛。γ-Producteria Halomonas argata DSM2581T是一株嗜盐细菌。埃托品水平根据外界盐分水平高度调节，但其新陈代谢和控制的整体情况还不是很清楚。除了在细胞适应嗜盐环境中的关键作用外，ectoine还可以用作酶的稳定剂，也可以作为皮肤和保健应用中的细胞保护剂，因此每年都会以吨级的规模在使用长链球藻作为生产菌的工业过程中生产。本文给出了长臂藻的全基因组序列(4 061 296个碱基)，包括鉴定和表征整个外生碱代谢的实验和分析，包括新发现的外生碱降解途径及其与外生碱合成的循环联系。胞外生碱(DOE)的降解是通过将胞外生碱(DOEA)水解成N-α-乙酰基-L-2，4-二氨基丁酸，然后去乙酰化成二氨基丁酸(DOE)进行的。在长链藻中，二氨基丁酸既可以流向天冬氨酸，也可以重新进入外生碱合成途径，形成外生碱合成和降解的循环。基因组比较表明，外生素的降解途径主要存在于不能合成外生碱的非嗜盐细菌中。根据得到的遗传和生化数据，推导出外生碱代谢的代谢通量模型，该模型可以用来理解长柄海棠在不同盐胁迫下的生存方式，并为以模型为驱动的工业外生碱生产的改进提供了基础。
The halophilic γ-proteobacterium Halomonas elongata DSM 2581T thrives at high salinity by synthesizing and accumulating the compatible solute ectoine. Ectoine levels are highly regulated according to external salt levels but the overall picture of its metabolism and control is not well understood. Apart from its critical role in cell adaptation to halophilic environments, ectoine can be used as a stabilizer for enzymes and as a cell protectant in skin and health care applications and is thus produced annually on a scale of tons in an industrial process using H. elongata as producer strain. This paper presents the complete genome sequence of H. elongata (4 061 296 bp) and includes experiments and analysis identifying and characterizing the entire ectoine metabolism, including a newly discovered pathway for ectoine degradation and its cyclic connection to ectoine synthesis. The degradation of ectoine (doe) proceeds via hydrolysis of ectoine (DoeA) to Nα-acetyl-l-2,4-diaminobutyric acid, followed by deacetylation to diaminobutyric acid (DoeB). In H. elongata, diaminobutyric acid can either flow off to aspartate or re-enter the ectoine synthesis pathway, forming a cycle of ectoine synthesis and degradation. Genome comparison revealed that the ectoine degradation pathway exists predominantly in non-halophilic bacteria unable to synthesize ectoine. Based on the resulting genetic and biochemical data, a metabolic flux model of ectoine metabolism was derived that can be used to understand the way H. elongata survives under varying salt stresses and that provides a basis for a model-driven improvement of industrial ectoine production.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
4.8
作者：
Androutsellis-Theotokis, A;Goldberg, NR;Rudnick, G
通讯作者：
Rudnick, G
影响因子：
4.4
作者：
Blatny, JM;Brautaset, T;Valla, S
通讯作者：
Valla, S
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD
影响因子：
4.8
作者：
Bolen, DW
通讯作者：
Bolen, DW