Increased complement C1q level marks active disease in human tuberculosis.
Increased complement C1q level marks active disease in human tuberculosis.
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补体 C1q 水平升高标志着人类结核病处于活动状态。
DOI：
10.1371/journal.pone.0092340
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
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作者：
Cai Y;Yang Q;Tang Y;Zhang M;Liu H;Zhang G;Deng Q;Huang J;Gao Z;Zhou B;Feng CG;Chen X
补体作为一种重要的宿主防御系统发挥作用，补体C5和C7参与了结核病的免疫病理过程。然而，对其他补体成分在结核病中的作用知之甚少。采用全基因组转录微阵列技术检测了结核病患者和对照者外周血单个核细胞中补体基因的表达。分别通过qRT-PCR和酶联免疫吸附试验进一步验证三种C1 q组分C1 qA、C1 qB和C1 qC的mRNA和蛋白水平。流式细胞术检测CD 14阳性细胞中C1 q表达的百分比。最后，定量结核性和非结核性胸膜炎胸水中C1 qC蛋白水平。与健康对照组和潜伏性结核感染者相比，活动性结核病患者外周血中C1 q表达显著增加。在活动性TB患者中，表达C1 q的CD 14阳性细胞的百分比显著增加。结核病患者外周血中c1 q表达与痰涂片阳性相关，且抗结核化疗后c1 q表达降低。值得注意的是，受试者操作特征分析表明，C1 qC mRNA水平在外周血中有效地区分活动性潜伏结核感染和健康对照。此外，胸腔积液中的C1 qC蛋白水平与其他炎症标志物（如IL-6和TNF-α）相比，在区分结核性和非结核性胸膜炎方面显示出更高的能力。C1 q表达与人类结核病活动性相关C1 q可作为区分活动性结核与潜伏性结核感染、结核性胸膜炎与非结核性胸膜炎的潜在诊断指标。
Complement functions as an important host defense system and complement C5 and C7 have been implicated in immunopathology of tuberculosis. However, little is known about the role of other complement components in tuberculosis. Complement gene expression in peripheral blood mononuclear cells of tuberculosis patients and controls were determined using whole genome transcriptional microarray assays. The mRNA and protein levels of three C1q components, C1qA, C1qB, and C1qC, were further validated by qRT-PCR and enzyme-linked immunosorbent assay, respectively. The percentages of C1q expression in CD14 positive cells were determined by flow cytometry. Finally, C1qC protein level was quantified in the pleural fluid of tuberculosis and non-tuberculosis pleurisy. C1q expression increases significantly in the peripheral blood of patients with active tuberculosis compared to healthy controls and individuals with latent TB infection. The percentage of C1q-expressing CD14 positive cells is significantly increased in active TB patients. C1q expression in the peripheral blood correlates with sputum smear positivity in tuberculosis patients and is reduced after anti-tuberculosis chemotherapy. Notably, receiver operating characteristic analysis showed that C1qC mRNA levels in peripheral blood efficiently discriminate active from latent tuberculosis infection and healthy controls. Additionally, C1qC protein level in pleural effusion shows improved power in discriminating tuberculosis from non-tuberculosis pleurisy when compared to other inflammatory markers, such as IL-6 and TNF-α. C1q expression correlates with active disease in human tuberculosis. C1q could be a potential diagnostic marker to discriminate active tuberculosis from latent tuberculosis infection as well as tuberculosis pleurisy from non-tuberculosis pleurisy.
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