Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo.
Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo.
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DOI：
10.1083/jcb.200912134
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发表时间：
2010-05-17
期刊：
影响因子：
--
通讯作者：
Wood W
中科院分区：
文献类型：
--
作者：
Stramer B;Moreira S;Millard T;Evans I;Huang CY;Sabet O;Milner M;Dunn G;Martin P;Wood W
微管臂调节细胞间的排斥，当果蝇胚胎中的血细胞相互接触时，微管臂将血细胞指向相反的方向。黑腹果蝇巨噬细胞是高度迁移的细胞，它们非常适合高分辨率的体内成像实验。通过表达荧光探针来揭示肌动蛋白和微管，我们可以观察到这两种细胞骨架网络的动态相互作用，因为巨噬细胞在活体内迁移并相互作用。我们发现，在持续运动之前，无论是对发育指导还是伤口线索做出反应，巨噬细胞都会组装一个极化的微管阵列，这些微管束成一个指南针样的手臂，似乎可以预测迁移的方向。每当细胞相互碰撞时，它们的微管臂在细胞-细胞排斥之前短暂对齐，我们表明，通过表达Spastin迫使微管解聚导致它们的极性缺陷和无法相互接触抑制。同样的是真实的轨道/扣突变体，表明这种微管结合蛋白的组装和/或微管臂的功能在极化迁移和接触排斥的关键作用。
A microtubule arm regulates cell–cell repulsion, pointing hemocytes in opposite directions when they contact each other in Drosophila embryos. Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.
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DOI：
10.1083/jcb.200301147
发表时间：
2003-06-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Janson ME;de Dood ME;Dogterom M
通讯作者：
Dogterom M
影响因子：
3.7
作者：
ABERCROMBIE, M;HEAYSMAN, JEM
通讯作者：
HEAYSMAN, JEM
影响因子：
7.8
作者：
Diamantopoulos, GS;Perez, F;Rickard, JE
通讯作者：
Rickard, JE
影响因子：
21.3
作者：
Krendel, M;Zenke, FT;Bokoch, GM
通讯作者：
Bokoch, GM
影响因子：
4.8
作者：
Ren, Y;Li, R;Busch, H
通讯作者：
Busch, H