Polyphenol (-)-epigallocatechin gallate during ischemia limits infarct size via mitochondrial K(ATP) channel activation in isolated rat hearts.
Polyphenol (-)-epigallocatechin gallate during ischemia limits infarct size via mitochondrial K(ATP) channel activation in isolated rat hearts.
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DOI：
10.3346/jkms.2010.25.3.380
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发表时间：
2010-03
影响因子：
4.5
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Song DK;Jang Y;Kim JH;Chun KJ;Lee D;Xu Z
茶多酚（-）-表没食子儿茶素没食子酸酯（EGCG），最丰富的儿茶素的绿色茶，似乎减轻心肌缺血/再灌注损伤。我们研究了ATP敏感性钾（KATP）通道参与EGCG诱导的心脏保护作用。离体大鼠心脏进行30分钟的区域缺血和2小时的再灌注。从缺血前10 min开始至缺血结束，再灌注40 min。非选择性KATP通道阻断剂格列本脲（GLI）和选择性线粒体KATP（mKATP）通道阻断剂5-羟基癸酸（HD）灌注在EGCG处理的心脏。在整个实验过程中，各组之间的冠状动脉流量和心脏动力学（包括心率、左室发展压、心率-血压乘积、+dP/dtmax和-dP/dtmin）无差异。EGCG治疗显著减少心肌梗死（14.5±2.5%，1 µM和4.0±1.7%，10 µM，P<0.001，对照组27.2±1.4%）。这种抗梗死作用被10 µM GLI完全消除（24.6± 1.5%，P<0.001 vs. EGCG）。类似地，100 µM HD也中止了EGCG的抗梗死作用（24.1± 1.2%，P<0.001 vs. EGCG）。这些数据支持KATP通道在EGCG诱导的心脏保护中的作用。mKATP通道在EGCG的心肌保护作用中起着重要作用。
Polyphenol (-)-epigallocatechin gallate (EGCG), the most abundant catechin of green tea, appears to attenuate myocardial ischemia/reperfusion injury. We investigated the involvement of ATP-sensitive potassium (KATP) channels in EGCG-induced cardioprotection. Isolated rat hearts were subjected to 30 min of regional ischemia and 2 hr of reperfusion. EGCG was perfused for 40 min, from 10 min before to the end of index ischemia. A nonselective KATP channel blocker glibenclamide (GLI) and a selective mitochondrial KATP (mKATP) channel blocker 5-hydroxydecanoate (HD) were perfused in EGCG-treated hearts. There were no differences in coronary flow and cardiodynamics including heart rate, left ventricular developed pressure, rate-pressure product, +dP/dtmax, and -dP/dtmin throughout the experiments among groups. EGCG-treatment significantly reduced myocardial infarction (14.5±2.5% in EGCG 1 µM and 4.0±1.7% in EGCG 10 µM, P<0.001 vs. control 27.2±1.4%). This anti-infarct effect was totally abrogated by 10 µM GLI (24.6±1.5%, P<0.001 vs. EGCG). Similarly, 100 µM HD also aborted the anti-infarct effect of EGCG (24.1±1.2%, P<0.001 vs. EGCG ). These data support a role for the KATP channels in EGCG-induced cardioprotection. The mKATP channels play a crucial role in the cardioprotection by EGCG.
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影响因子：
9.5
作者：
STEENBERGEN, C;FRALIX, TA;MURPHY, E
通讯作者：
MURPHY, E
影响因子：
3.3
作者：
Sano, J;Inami, S;Mizuno, K
通讯作者：
Mizuno, K
影响因子：
9.5
作者：
Lochner, A;Genade, S;Moolman, JA
通讯作者：
Moolman, JA
DOI：
10.1152/ajpheart.00493.2007
发表时间：
2007-09-01
影响因子：
4.8
作者：
Shinohara, Tetsuji;Takahashi, Naohiko;Yoshimatsu, Hironobu
通讯作者：
Yoshimatsu, Hironobu
影响因子：
120.7
作者：
Kuriyama, Shinichi;Shimazu, Taichi;Tsuji, Ichiro
通讯作者：
Tsuji, Ichiro