Recombinant adenovirus expressing the haemagglutinin of Peste des petits ruminants virus (PPRV) protects goats against challenge with pathogenic virus; a DIVA vaccine for PPR.
Recombinant adenovirus expressing the haemagglutinin of Peste des petits ruminants virus (PPRV) protects goats against challenge with pathogenic virus; a DIVA vaccine for PPR.
复制标题
表达小反刍兽疫病毒（PPRV）血凝素的重组腺病毒可保护山羊免受致病病毒的攻击；针对 PPR 的 DIVA 疫苗。
DOI：
10.1186/1297-9716-45-24
复制
发表时间：
2014-02-26
影响因子：
4.4
通讯作者：
Taylor G
中科院分区：
文献类型：
--
作者：
Herbert R;Baron J;Batten C;Baron M;Taylor G
小反刍动物害虫病毒（PPRV）是一种麻疹病毒，可在绵羊和山羊中引起严重疾病，其特征是发热、气性肠炎和胃炎。在不发达国家，该病的社会经济负担正在增加，贫穷的牲畜饲养者受到的影响最大。目前的疫苗由细胞培养的PPRV减毒株组成，其诱导的抗体谱与自然感染诱导的相似。生产一种能够区分受感染动物和接种疫苗动物的疫苗（DIVA）将有利于小反刍兽疫的控制和根除规划，特别是在根除运动的后期阶段和该疾病不流行的国家。为了制造一种能够使感染动物与已接种疫苗的动物区分开来的疫苗（DIVA疫苗），我们在山羊身上评估了表达PPRV F和H蛋白的重组禽痘（FP）和复制缺陷重组人腺病毒5 （Ad）的免疫原性。与fp载体疫苗相比，Ad构建体诱导了更高水平的病毒特异性抗体和中和抗体，并引发了更多数量的CD8+ T细胞。重要的是，单剂量的Ad- h，无论是否添加表达羊粒细胞巨噬细胞集落刺激因子和/或羊白细胞介素-2的Ad，不仅诱导了强抗体和细胞介导的免疫，而且在接种后4个月完全保护山羊免受强毒PPRV的攻击。因此，复制缺陷Ad-H提供了一种有效的DIVA疫苗的可能性。
Peste des petits ruminants virus (PPRV) is a morbillivirus that can cause severe disease in sheep and goats, characterised by pyrexia, pneumo-enteritis, and gastritis. The socio-economic burden of the disease is increasing in underdeveloped countries, with poor livestock keepers being affected the most. Current vaccines consist of cell-culture attenuated strains of PPRV, which induce a similar antibody profile to that induced by natural infection. Generation of a vaccine that enables differentiation of infected from vaccinated animals (DIVA) would benefit PPR control and eradication programmes, particularly in the later stages of an eradication campaign and for countries where the disease is not endemic. In order to create a vaccine that would enable infected animals to be distinguished from vaccinated ones (DIVA vaccine), we have evaluated the immunogenicity of recombinant fowlpox (FP) and replication-defective recombinant human adenovirus 5 (Ad), expressing PPRV F and H proteins, in goats. The Ad constructs induced higher levels of virus-specific and neutralising antibodies, and primed greater numbers of CD8+ T cells than the FP-vectored vaccines. Importantly, a single dose of Ad-H, with or without the addition of Ad expressing ovine granulocyte macrophage colony-stimulating factor and/or ovine interleukin-2, not only induced strong antibody and cell-mediated immunity but also completely protected goats against challenge with virulent PPRV, 4 months after vaccination. Replication-defective Ad-H therefore offers the possibility of an effective DIVA vaccine.
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影响因子：
5.1
作者：
Geutskens, SB;van der Eb, MM;Hoeben, RC
通讯作者：
Hoeben, RC
影响因子：
5.5
作者：
JONES, L;GIAVEDONI, L;YILMA, T
通讯作者：
YILMA, T
影响因子：
5
作者：
Abaitua, F;Rodríguez, JR;Esteban, M
通讯作者：
Esteban, M
影响因子：
6.4
作者：
Gans, Hayley A.;Yasukawa, Linda L.;Arvin, Ann M.
通讯作者：
Arvin, Ann M.
影响因子：
2.8
作者：
Alvarez-Lajonchere, Liz;Amador-Canizares, Yalena;Duenas-Carrera, Santiago
通讯作者：
Duenas-Carrera, Santiago