Characterizing expression pattern of Six2Cre during mouse craniofacial development.

Characterizing expression pattern of Six2Cre during mouse craniofacial development.
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DOI:
10.1002/dvg.23516
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发表时间:
2023-09
期刊:
Genesis (New York, N.Y. : 2000)
影响因子:
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其他
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颅面发育是一个涉及不同细胞群体的复杂过程。已经开发了各种转基因Cre系以便于研究特定组织中的基因功能。在这项研究中,我们的特点Six2Cre小鼠在颅面发育的多个阶段的表达模式。我们的数据显示,Six2Cre谱系细胞主要存在于额骨,下颌骨和次生腭。利用免疫染色方法,我们发现Six2Cre触发的报告基因与Runx2共表达。总之,我们的数据表明Six2Cre可以用于研究小鼠模型中腭发育和成骨过程中的基因功能。
Craniofacial development is a complex process involving diverse cell populations. Various transgenic Cre lines have been developed to facilitate studying gene function in specific tissues. In this study, we have characterized the expression pattern of Six2Cre mice at multiple stages during craniofacial development. Our data revealed that Six2Cre lineage cells are predominantly present in frontal bone, mandible, and secondary palate. Using immunostaining method, we found that Six2Cre triggered reporter is co-expressed with Runx2. In summary, our data showed Six2Cre can be used to study gene function during palate development and osteogenesis in mouse models.
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