The influence of amino acids structure on their anaerobic digestion and the strategy to enhance biotransformation of refractory ones
The influence of amino acids structure on their anaerobic digestion and the strategy to enhance biotransformation of refractory ones
复制标题
氨基酸结构对其厌氧消化的影响及促进难降解氨基酸生物转化的策略
DOI：
10.1016/j.cej.2020.128169
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发表时间：
2021-04
影响因子：
15.1
通讯作者：
Yinguang Chen
中科院分区：
文献类型：
--
作者：
Meng Wang;Tong Yu;Yang Huo;Yinguang Chen
蛋白质是有机废物的重要组成部分，在厌氧消化（AD）过程中首先水解为氨基酸（AA）。然而，到目前为止，AA 结构对其 AD 的影响尚未有记录。本文研究了AA结构对其两相AD的影响以及增强难降解AA的生物转化的策略。首先，比较了 19 种水溶性 AA 的 AD 性能。除甘氨酸 (284.1 mL/gCODadd) 外，极性 AA 的甲烷产率高于非极性氨基酸 (162.3–308.1 对比 10.7–140.5 mL/gCODadd)。进一步的研究表明，极性氨基酸产生甲烷的顺序根据其碳原子数为4-C < 6-C < 3-C < 5-C，而非极性氨基酸产生甲烷的顺序为长链氨基酸（5至11-C）<短链氨基酸（2至3-C）。长链非极性氨基酸根据其官能团的不同，其甲烷产率依次为：脯氨酸>烷基AA>Ph AA。机理研究表明，AAs结构与除丙酸之外的VFA含量之间的关系与AAs结构对甲烷产率的影响一致。难降解AA的AD系统中的微生物具有较高的细胞表面疏水性（CSH）和较低的细胞膜通透性（CMP），导致酶活性较低、氨基酸降解以及VFA生成较低，从而导致甲烷产量较少。最后，研究了提高难降解AA生物转化的策略，通过应用鼠李糖脂改变消化系统中的CSH和CMP，甲烷产率提高了139.8%。这些发现阐明了AA结构与AD性能之间的关系，为改善难治性AA的生物转化提供了新思路。
Protein is an important component of organic waste, and it is first hydrolyzed to amino acids (AAs) during anaerobic digestion (AD). Until now, however, the effect of AAs structure on their AD has never been documented. In this paper the influence of AAs structure on their two-phase AD and the strategy to enhance biotransformation of refractory ones were investigated. Firstly, the AD performance of nineteen water-soluble AAs was compared. The methane yield of polar AAs was higher than that of non-polar ones (162.3–308.1 verse 10.7–140.5 mL/gCODadd) except glycine (284.1 mL/gCODadd). Further studies showed that the order of methane production with polar AAs, according to their carbon number, was 4-C < 6-C < 3-C < 5-C, while that with non-polar AAs was long-chain AAs (5 to 11-C) < short-chain AAs (2 to 3-C). The methane yield of non-polar ones with long-chain, according to their functional groups, was followed: proline > Alkyl AAs > Ph AAs. The mechanism investigation revealed the relationship between the structure of AAs and the amount of VFAs excluding propionate was consistent with the influence of AAs structure on methane yield. The microbes in AD system of refractory AAs had higher cell surface hydrophobicity (CSH) and lower cell membrane permeability (CMP), which led to lower enzyme activities and amino acid degradation as well as VFAs generation, and thus less methane production. Finally, the strategy to increase the biotransformation of refractory AAs was studied, and the methane yield was increased up to 139.8% by the application of rhamnolipid to change CSH and CMP in the digestion system. These findings elucidated the relationship between AAs structure and AD performance and provided new ideas to improve the biotransformation of refractory AAs.
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DOI：
10.1016/j.bjm.2016.04.005
发表时间：
2016-07
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Peralta GH;Bergamini CV;Hynes ER
通讯作者：
Hynes ER
影响因子：
11.4
作者：
Yuan, HY;Chen, YG;Gu, GW
通讯作者：
Gu, GW
低温预处理对含盐废活性污泥理化性质、厌氧消化有机物水解及甲烷产率的影响
影响因子：
11.4
作者：
Biswal, Basanta Kumar;Huang, Hao;Wu, Di
通讯作者：
Wu, Di
低浓度鼠李糖脂对亲水和疏水表面铜绿假单胞菌 ATCC 9027 吸附的影响
影响因子：
13.6
作者：
Zhong Hua;Jiang Yongbing;Zeng Guangming;Liu Zhifeng;Liu Liuxia;Liu Yang;Yang Xin;Lai Mingyong;He Yibin
通讯作者：
He Yibin
鼠李糖脂介导的疏水性有机化合物生物降解机制
影响因子：
9.8
作者：
Zeng Zhuotong;Liu Yang;Zhong Hua;Xiao Rong;Zeng Guangming;Liu Zhifeng;Cheng Min;Lai Cui;Zhang Chen;Liu Guansheng;Qin Lei
通讯作者：
Qin Lei