The effects of transient starvation persist through direct interactions between CaMKII and ether-a-go-go K+ channels in C. elegans males.
The effects of transient starvation persist through direct interactions between CaMKII and ether-a-go-go K+ channels in C. elegans males.
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DOI：
10.1016/j.neuroscience.2010.12.002
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发表时间：
2011-02-23
期刊：
影响因子：
3.3
通讯作者：
Garcia, L. R.
中科院分区：
文献类型：
--
作者：
LeBoeuf, B.;Guo, X.;Garcia, L. R.
长期营养限制因其对寿命的积极影响而受到广泛研究。然而，人们对于短暂的饥饿会产生多大的持久影响却知之甚少。在这项研究中，我们利用遗传学、生物化学、药理学和行为分析来表明，经过一段有限的饥饿后，egl-2编码的ether-a-go-go (EAG) K+通道的合成及其C端由unc-43编码的CaMKII修饰对秀丽隐杆线虫雄性性行为具有持久影响。禁食后诱导的 EGL-2 和 UNC-43 相互作用，维持参与性行为的肌肉兴奋性降低。在年轻成年男性中，痉挛性收缩发生在胆碱能激活的性肌肉中，这些肌肉缺乏功能性的 unc-103 编码的 ERG 样 K+ 通道。通过让 unc-103 突变成年男性挨饿几个小时来促进 EGL-2 和 UNC-43 的相互作用，可以减少多天内的痉挛性肌肉收缩。尽管成年早期的短暂饥饿具有抑制突变引起的肌肉收缩的激效效应，但这种治疗降低了年轻野生型雄性与喂养良好的雄性后代竞争的能力。
Prolonged nutrient limitation has been extensively studied due to its positive effects on life span. However, less is understood of how brief periods of starvation can have lasting consequences. In this study, we use genetics, biochemistry, pharmacology and behavioral analysis to show that after a limited period of starvation, the synthesis of egl-2-encoded ether-a-go-go (EAG) K+ channels and its C-terminal modifications by unc-43-encoded CaMKII have a perduring effect on C. elegans male sexual behavior. EGL-2 and UNC-43 interactions, induced after food deprivation, maintain reduced excitability in muscles involved in sex. In young adult males, spastic contractions occur in cholinergic-activated sex muscles that lack functional unc-103-encoded ERG-like K+ channels. Promoting EGL-2 and UNC-43 interactions in unc-103 mutant adult males by starving them for a few hours reduce spastic muscle contractions over multiple days. Although transient starvation during early adulthood has a hormetic effect of suppressing mutation-induced muscle contractions, the treatment reduces the ability of young wild-type males to compete with well-fed cohorts in siring progeny.
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DOI：
10.1126/science.1173635
发表时间：
2009-07-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colman RJ;Anderson RM;Johnson SC;Kastman EK;Kosmatka KJ;Beasley TM;Allison DB;Cruzen C;Simmons HA;Kemnitz JW;Weindruch R
通讯作者：
Weindruch R
影响因子：
2.9
作者：
ERNEST, MJ
通讯作者：
ERNEST, MJ
DOI：
10.1111/j.1749-6632.1999.tb11297.x
发表时间：
1999-01-01
期刊：
MOLECULAR AND FUNCTIONAL DIVERSITY OF ION CHANNELS AND RECEPTORS
影响因子：
--
作者：
Ganetzky, B;Robertson, GA;Titus, SA
通讯作者：
Titus, SA
影响因子：
7.8
作者：
Burger, Joep M. S.;Hwangbo, Dae Sung;Promislow, Daniel E. L.
通讯作者：
Promislow, Daniel E. L.
DOI：
10.1089/dna.1.1989.8.481
发表时间：
1989-09-01
期刊：
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY
影响因子：
--
作者：
JONES, D;DIXON, DK;CANDIDO, EPM
通讯作者：
CANDIDO, EPM