Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching.
Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching.
复制标题
DOI：
10.3791/57665
复制
发表时间：
2018-05-17
期刊：
影响因子：
--
通讯作者：
Alivisatos AP
中科院分区：
文献类型：
--
作者：
Hauwiller MR;Ondry JC;Alivisatos AP
石墨烯液体细胞电子显微镜能够观察液体环境中发生的纳米级化学转变和动力学。本手稿通过金纳米晶体蚀刻的石墨烯液体电池透射电子显微镜（TEM）实验的例子描述了制造石墨烯液体电池的过程。制造石墨烯液体电池的方案包括用化学气相沉积石墨烯涂覆金、多孔碳 TEM 网格，然后使用这些石墨烯涂覆的网格将液体封装在两个石墨烯表面之间。这些含有感兴趣的纳米材料的液体在电子显微镜中成像，以观察纳米级过程的动态，在本例中是金纳米棒的氧化蚀刻。通过控制电子束剂量率（调节液体单元中的蚀刻物质），可以更好地理解原子如何从纳米晶体中去除以形成不同面和形状的基本机制。石墨烯液池TEM具有空间分辨率高、与传统TEM支架兼容以及研究小组启动成本低等优点。目前的限制包括精细的样品制备、缺乏流动能力以及依赖电子束产生的辐射分解产物来诱导反应。随着进一步的发展和控制，石墨烯液体电池可能成为纳米材料和生物学中普遍存在的技术，并且已经被用于研究单颗粒水平上纳米材料在液体中的生长、蚀刻和自组装过程的控制机制。
Graphene liquid cell electron microscopy provides the ability to observe nanoscale chemical transformations and dynamics as the reactions are occurring in liquid environments. This manuscript describes the process for making graphene liquid cells through the example of graphene liquid cell transmission electron microscopy (TEM) experiments of gold nanocrystal etching. The protocol for making graphene liquid cells involves coating gold, holey-carbon TEM grids with chemical vapor deposition graphene and then using those graphene-coated grids to encapsulate liquid between two graphene surfaces. These pockets of liquid, with the nanomaterial of interest, are imaged in the electron microscope to see the dynamics of the nanoscale process, in this case the oxidative etching of gold nanorods. By controlling the electron beam dose rate, which modulates the etching species in the liquid cell, the underlying mechanisms of how atoms are removed from nanocrystals to form different facets and shapes can be better understood. Graphene liquid cell TEM has the advantages of high spatial resolution, compatibility with traditional TEM holders, and low start-up costs for research groups. Current limitations include delicate sample preparation, lack of flow capability, and reliance on electron beam-generated radiolysis products to induce reactions. With further development and control, graphene liquid cell may become a ubiquitous technique in nanomaterials and biology, and is already being used to study mechanisms governing growth, etching, and self-assembly processes of nanomaterials in liquid on the single particle level.
登录
查看更多内容
影响因子：
56.9
作者：
Liao, Hong-Gang;Cui, Likun;Zheng, Haimei
通讯作者：
Zheng, Haimei
影响因子：
64.8
作者：
Mirkin, CA;Letsinger, RL;Storhoff, JJ
通讯作者：
Storhoff, JJ
影响因子：
10.8
作者：
Chen, Qian;Smith, Jessica M.;Alivisatos, A. Paul
通讯作者：
Alivisatos, A. Paul
影响因子：
1.2
作者：
Niu, Kai-Yang;Liao, Hong-Gang;Zheng, Haimei
通讯作者：
Zheng, Haimei
影响因子：
10.8
作者：
Cho, Hoduk;Jones, Matthew R.;Alivisatos, A. Paul
通讯作者：
Alivisatos, A. Paul