Intestinal microbiota is different in women with preterm birth: results from terminal restriction fragment length polymorphism analysis.
Intestinal microbiota is different in women with preterm birth: results from terminal restriction fragment length polymorphism analysis.
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早产女性的肠道菌群不同：终末限制片段长度多态性分析的结果。
DOI：
10.1371/journal.pone.0111374
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Saito S
中科院分区：
文献类型：
--
作者：
Shiozaki A;Yoneda S;Yoneda N;Yonezawa R;Matsubayashi T;Seo G;Saito S
早产是围产期发病和死亡的主要原因。利用培养法或分子鉴定的研究表明细菌性阴道病是早产的危险因素之一。然而，尽管阴道微生物群在怀孕期间发生了变化，但尚未有使用分子技术报道早产与肠道微生物群之间的关联。我们的目的是阐明早产妇女和未早产妇女肠道和阴道微生物群的差异。采用聚合酶链反应从粪便和阴道DNA中扩增出16S核糖体核糖核酸基因。利用末端限制性片段长度多态性（T-RFLP），我们比较了三组孕妇肠道和阴道菌群的操作分类单位水平：分娩足月婴儿未早产的孕妇（非PTL组）（n = 20），早产但分娩足月婴儿的孕妇（PTL组）（n = 11）和早产的孕妇（PTB组）（n = 10）。与非ptl组相比，PTB组肠道微生物群中梭菌群XVIII亚群、梭菌群IV亚群、梭菌群XIVa亚群和拟杆菌群水平显著降低，乳酸杆菌群水平显著升高。PTB组Clostridium XVIII亚群和Clostridium XIVa亚群水平显著低于PTL组，且PTL组Clostridium XVIII亚群和XIVa亚群水平显著低于非PTL组。然而，三组之间阴道微生物群没有显著差异。使用T-RFLP方法发现PTB组的肠道微生物群与非ptl组不同。
Preterm birth is a leading cause of perinatal morbidity and mortality. Studies using a cultivation method or molecular identification have shown that bacterial vaginosis is one of the risk factors for preterm birth. However, an association between preterm birth and intestinal microbiota has not been reported using molecular techniques, although the vaginal microbiota changes during pregnancy. Our aim here was to clarify the difference in intestinal and vaginal microbiota between women with preterm birth and women without preterm labor. 16S ribosomal ribonucleic acid genes were amplified from fecal and vaginal DNA by polymerase chain reaction. Using terminal restriction fragment length polymorphism (T-RFLP), we compared the levels of operational taxonomic units of both intestinal and vaginal flora among three groups: pregnant women who delivered term babies without preterm labor (non-PTL group) (n = 20), those who had preterm labor but delivered term babies (PTL group) (n = 11), and those who had preterm birth (PTB group) (n = 10). Significantly low levels of Clostridium subcluster XVIII, Clostridium cluster IV, Clostridium subcluster XIVa, and Bacteroides, and a significantly high level of Lactobacillales were observed in the intestinal microbiota in the PTB group compared with those in the non-PTL group. The levels of Clostridium subcluster XVIII and Clostridium subcluster XIVa in the PTB group were significantly lower than those in the PTL group, and these levels in the PTL group were significantly lower than those in non-PTL group. However, there were no significant differences in vaginal microbiota among the three groups. Intestinal microbiota in the PTB group was found to differ from that in the non-PTL group using the T-RFLP method.
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
3.7
作者：
Aagaard K;Riehle K;Ma J;Segata N;Mistretta TA;Coarfa C;Raza S;Rosenbaum S;Van den Veyver I;Milosavljevic A;Gevers D;Huttenhower C;Petrosino J;Versalovic J
通讯作者：
Versalovic J
影响因子：
7.1
作者：
Myhre, Ronny;Brantsoeter, Anne Lise;Jacobsson, Bo
通讯作者：
Jacobsson, Bo
不良妊娠结局（APOs）和牙周病：致病机制
影响因子：
6.7
作者：
Madianos, Phoebus N.;Bobetsis, Yiorgos A.;Offenbacher, Steven
通讯作者：
Offenbacher, Steven
影响因子：
9.4
作者：
Fredricks, David N.;Fiedler, Tina L.;Marrazzo, Jeanne M.
通讯作者：
Marrazzo, Jeanne M.