Selective fluorogenic derivatization of a peptide nucleic acid trimer with naphthalene-2,3-dicarboxaldehyde.
Selective fluorogenic derivatization of a peptide nucleic acid trimer with naphthalene-2,3-dicarboxaldehyde.
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肽核酸三聚体与萘-2,3-二甲醛的选择性荧光衍生化。
DOI：
10.1016/s0731-7085(96)01927-9
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发表时间：
1997
影响因子：
3.4
通讯作者：
R. Borchardt
中科院分区：
文献类型：
--
作者：
M. Nerurkar;M. Rose;J. Stobaugh;R. Borchardt
本文研究了肽核酸（PNA）三聚体在氰化物存在下用萘二甲醛（NDA/CN）荧光衍生化后的反相高效液相色谱。在衍生前浓度低至48.9 ng ml-1的含有PNA三聚体的细胞匀浆样品中测定痕量水平的PNA三聚体。样品预处理操作包括通过超滤实现的脱蛋白步骤，然后进行荧光衍生化（NDA/CN）。随后，为了实现足够的选择性，在通过荧光检测进行定量之前，对荧光标记的PNA进行高效阴离子交换色谱。在样品预处理和分离生物样品中衍生的PNA三聚体过程中遇到的各种问题，并进行了讨论。
The reversed-phase high-performance liquid chromatography of a Peptide Nucleic Acid (PNA) trimer has been studied after its preseparation fluorogenic derivatization with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide (NDA/CN). Trace levels of the PNA trimer were determined in cell homogenate samples containing the PNA trimer at prederivatization concentrations as low as 48.9 ng ml−1. The sample pretreatment operations included a deproteination step, achieved by ultra-filtration, followed by fluorogenic derivatization (NDA/CN). Subsequently, to achieve adequate selectivity, the fluorescently labeled PNA was subjected to high performance anion exchange chromatography prior to quantitation via fluorescence detection. The various problems encountered during sample pretreatment and separation of derivatized PNA trimer in biological samples are presented and discussed.
LC-MS 兼容的 HPLC 分离异生素及其 I 相和 II 相代谢物：同步阴离子交换和反相色谱。
影响因子：
1.3
作者：
Johnston,JJ;Draper,WM;Stephens,RD
通讯作者：
Stephens,RD
用萘-2,3-二甲醛/氰化物对肽进行荧光衍生化：产量优化及其在测定亮氨酸脑啡肽加标人血浆样品中的应用。
影响因子：
3.4
作者：
deMontigny,P;Riley,CM;Sternson,LA;Stobaugh,JF
通讯作者：
Stobaugh,JF
阿片肽的多维液相色谱：荧光标记、保留预测和分离优化。
影响因子：
3.4
作者：
Nicholson,LM;Patel,HB;Kristjansson,F;CrowleyJr,SC;Dave,K;Stobaugh,JF;Riley,CM
通讯作者：
Riley,CM