Expression of a Brassica napus heme oxygenase confers plant tolerance to mercury toxicity.
Expression of a Brassica napus heme oxygenase confers plant tolerance to mercury toxicity.
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甘蓝型油菜血红素加氧酶的表达赋予植物对汞毒性的耐受性。
DOI：
10.1111/j.1365-3040.2011.02279.x
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Shen Q, Jiang M, Li H, Che LL, Yang ZM*
中科院分区：
文献类型：
--
作者：
Shen Q, Jiang M, Li H, Che LL, Yang ZM*
植物血红素加氧酶（HO）调节光敏色素的生物合成，光敏色素的生物合成与植物的光接受和形态建成有关。最近的研究表明，植物HO还调节许多其他生理过程，包括对环境刺激的反应。为了阐明HO调控植物对重金属胁迫的适应机制，从油菜中分离了3个新的HO基因，并分析了它们的表达模式。同源性分析表明，这3个BnHO与拟南芥AtHO 1 -3同源性较高。为了研究BnHO是否调节植物对汞毒性的耐受性，我们构建了B。转BnHO-1基因油菜植株。在汞胁迫下，转基因植株的生物量比未转基因植株高1.41-1.59倍。然而，BnHO-1的过表达导致汞在某些转化株系中的积累比未转化株中少。与未转基因植株相比，转基因植株表现出较低的活性氧丰度和减弱的氧化损伤。我们从B中克隆了BnHO-1的启动子序列。油菜。分析表明，1119 bp的片段包含一个保守的镉响应元件（CdRE）和其他响应多种环境刺激。 烟草叶片中的瞬时表达对重金属（Zn、Cu、Pb、Hg和Cd）表现出不同的响应。
Plant heme oxygenases (HOs) regulate biosynthesis of phytochrome which accounts for photo-acceptance and -morphogenesis. Recent studies have demonstrated that plant HOs also regulate many other physiological processes including response to environmental stimuli. To elucidate the mechanism by which HOs regulate plant adaptation to heavy metal exposure, three novel HOs genes were isolated from rapeseed (Brassica napus) and their expression patterns were analysed. Alignment of deduced protein sequences revealed that the three BnHOs share high identity with their corresponding orthologos (AtHO1-3) from Arabidopsis. To investigate whether the BnHO regulates plant tolerance to Hg toxicity, we constructed B. napus transgenic plants overexpressing BnHO-1. Under Hg stress, the transgenic plants had 1.41-1.59 folds higher biomass than the untransformants. However, overexpression of BnHO-1 resulted in less accumulation of Hg in some lines of transformants than in untransformants. The transgenic plants show lower abundance of reactive oxygen species and attenuated oxidative injury compared with the untransgenic plants. We cloned the promoter sequences of BnHO-1 from B. napus. Analysis revealed that the 1119 bp fragment contains a conserved Cd responsive element (CdRE) and others responding to multiple environmental stimuli. Transient expression in tobacco leaves showed differential responses to heavy metals (Zn, Cu, Pb, Hg and Cd).
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影响因子：
2.7
作者：
H. Chen;R. S. Nelson;Sherwood Jl
通讯作者：
H. Chen;R. S. Nelson;Sherwood Jl
影响因子：
11.4
作者：
Ye Wu;Shuxiao Wang;D. Streets;J. Hao;M. Chan;Jingkun Jiang
通讯作者：
Ye Wu;Shuxiao Wang;D. Streets;J. Hao;M. Chan;Jingkun Jiang
影响因子：
3
作者：
Dekker, J;Hargrove, M
通讯作者：
Hargrove, M
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
7.4
作者：
Cobbett, CS
通讯作者：
Cobbett, CS