Impact of the dual defence system of Plantago lanceolata (Plantaginaceae) on performance, nutrient utilisation and feeding choice behaviour of Amata mogadorensis larvae (Lepidoptera, Erebidae).
Impact of the dual defence system of Plantago lanceolata (Plantaginaceae) on performance, nutrient utilisation and feeding choice behaviour of Amata mogadorensis larvae (Lepidoptera, Erebidae).
复制标题
车前子（车前草科）的双重防御系统对 Amata mogadorensis 幼虫（鳞翅目，Erebidae）的性能、养分利用和取食选择行为的影响。
DOI：
10.1016/j.jinsphys.2015.08.006
复制
发表时间：
2015
影响因子：
2.2
通讯作者：
C. Müller
中科院分区：
文献类型：
--
作者：
Helga Pankoke;René Gehring;C. Müller
环烯醚萜苷是植物防御化合物，对不适应的食草动物具有潜在的有害影响。一些植物物种具有水解环烯醚萜苷并由此释放蛋白质变性糖苷配基的β-葡糖苷酶。为了验证环烯醚萜苷和植物β-葡萄糖苷酶形成双重防御系统的假设，我们使用了Plantago lanceolata和一种多食性毛虫。为了分析环烯醚萜苷浓度和β-葡萄糖苷酶活性的叶龄依赖性差异对昆虫性能的影响，将老叶或嫩叶冷冻干燥并掺入人工饲料中或新鲜提供给幼虫。我们测定了幼虫的消耗率和同化氮的量。此外，我们定量了人工饲料和新鲜叶片中的β-葡萄糖苷酶活性以及幼虫取食新鲜叶片时摄入和排泄的环烯醚萜苷的量。与新鲜叶片相比，幼虫在人工饲料中生长更快，幼虫增重与氮的吸收量呈正相关。当喂养新鲜的嫩叶，幼虫甚至失去了重量和排泄物中的环烯醚萜苷的完整的粪便，表明这些化合物的水解可能会干扰氮同化和受损的幼虫的生长只有微小的比例。为了从环烯醚萜苷的威慑作用中解开生理效应，我们进行了双重选择摄食试验。幼叶，其甲醇提取物和纯梓醇减少幼虫喂养相比，各自的控制，而桃叶珊瑚苷幼虫消费没有影响。我们的结论是，双重防御系统的P。由环烯醚萜苷和β-葡萄糖苷酶组成的lanceolatase干扰环烯醚萜苷的营养利用-水解，并且还以浓度和物质特异性的方式介导幼虫的摄食行为。
Iridoid glycosides are plant defence compounds with potentially detrimental effects on non-adapted herbivores. Some plant species possess β-glucosidases that hydrolyse iridoid glycosides and thereby release protein-denaturing aglycones. To test the hypothesis that iridoid glycosides and plant β-glucosidases form a dual defence system, we usedPlantago lanceolataand a polyphagous caterpillar species. To analyse the impact of leaf-age dependent differences in iridoid glycoside concentrations and β-glucosidase activities on insect performance, old or young leaves were freeze-dried and incorporated into artificial diets or were provided freshly to the larvae. We determined larval consumption rates and the amounts of assimilated nitrogen. Furthermore, we quantified β-glucosidase activities in artificial diets and fresh leaves and the amount of iridoid glycosides that larvae feeding on fresh leaves ingested and excreted. Compared to fresh leaves, caterpillars grew faster on artificial diets, on which larval weight gain correlated positively to the absorbed amount of nitrogen. When feeding fresh young leaves, larvae even lost weight and excreted only minute proportions of the ingested iridoid glycosides intact with the faeces, indicating that the hydrolysis of these compounds might have interfered with nitrogen assimilation and impaired larval growth. To disentangle physiological effects from deterrent effects of iridoid glycosides, we performed dual choice feeding assays. Young leaves, their methanolic extracts and pure catalpol reduced larval feeding in comparison to the respective controls, while aucubin had no effect on larval consumption. We conclude that the dual defence system ofP. lanceolataconsisting of iridoid glycosides and β-glucosidases interferes with the nutrient utilisationviathe hydrolysis of iridoid glycosides and also mediates larval feeding behaviour in a concentration- and substance-specific manner.
DOI：
10.1073/pnas.1202111109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dobler, Susanne;Dalla, Safaa;Agrawal, Anurag A.
通讯作者：
Agrawal, Anurag A.
毒素释放β-葡萄糖苷酶和植物环烯醚萜苷之间的相互作用损害多面性毛毛虫Grammia incorrupta（Arctiidae）的幼虫发育
影响因子：
3.8
作者：
Pankoke H;Bowers D;Dobler S.
通讯作者：
Dobler S.