High-throughput development of genome-wide locus-specific informative SSR markers in wheat
High-throughput development of genome-wide locus-specific informative SSR markers in wheat
复制标题
小麦全基因组位点特异性信息 SSR 标记的高通量开发
DOI：
10.1007/s11427-016-0252-x
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发表时间：
2016-12
影响因子：
9.1
通讯作者：
Li Tao
中科院分区：
文献类型：
--
作者：
Li Lei;Sun Fayu;Wu Di;Zhen Fei;Bai Guihua;Gao Derong;Li Tao
Although simple sequence repeat (SSR) markers are not new, they are still useful and often used markers in molecular mapping and marker-assisted breeding, particularly in developing countries. However, locus-specific SSR markers could be more useful and informative in wheat breeding and genetic studies. In the present study, 221,911 locus-specific SSR markers were designed. Verification of polymorphisms showed that the proportion of polymorphic markers increases with an increase in SSR size. Evaluation of the polymorphic information content (PIC) of the SSR markers in a panel of 12 wheat accessions showed that PIC also increases with an increase in SSR size. Alternative locus-specific SSR markers for known QTLs and the distributions of SSR markers and single-nucleotide polymorphisms (SNPs) from the Axiom 820K SNP array were also analyzed in this study. A total of 1,037,987 SSRs were identified from a 10.2 Gb reference sequence of the hexaploid wheat cultivar Chinese Spring (CSS). SSRs were found to be considerably abundant with an average density of SSR per 9.8 kb (Table S1A in Sup-
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影响因子：
7
作者：
Schuler, GD
通讯作者：
Schuler, GD
节节山羊草基因组序列揭示了小麦适应的基因库
影响因子：
64.8
作者：
Jia, Jizeng;Zhao, Shancen;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
--
作者：
S. Rozen;H. Skaletsky
通讯作者：
S. Rozen;H. Skaletsky
影响因子：
3.7
作者：
Poland JA;Brown PJ;Sorrells ME;Jannink JL
通讯作者：
Jannink JL
影响因子：
64.8
作者：
Ling, Hong-Qing;Zhao, Shancen;Wang, Jun
通讯作者：
Wang, Jun