The pore-forming protein (perforin) of cytolytic T lymphocytes is immunologically related to the components of membrane attack complex of complement through cysteine-rich domains.
The pore-forming protein (perforin) of cytolytic T lymphocytes is immunologically related to the components of membrane attack complex of complement through cysteine-rich domains.
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DOI：
10.1084/jem.164.6.2077
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发表时间：
1986-12-01
期刊：
影响因子：
--
通讯作者：
Cohn ZA
中科院分区：
文献类型：
--
作者：
Young JD;Liu CC;Leong LG;Cohn ZA
最近描述了补体第九组分 (C9) 和淋巴细胞成孔蛋白（PFP，穿孔素）之间的结构、功能和免疫学相似性 (8-10)。此处显示 PFP 与人补体膜攻击复合物 (MAC) 的所有其他成分（即 C5b-6、C7、C8 和 C9）在免疫学上相关。针对纯化的人 C5b-6、C7、C8 或 C9 产生的多克隆抗体与 MAC 的其他成分以及小鼠淋巴细胞 PFP 发生反应。人补体蛋白和小鼠淋巴细胞 PFP 共享的抗原表位仅限于富含半胱氨酸的结构域。当用作免疫原时，只有已被还原和烷基化的补体蛋白才会引发交叉反应抗体的产生。补体成分或淋巴细胞 PFP 的非还原形式既不与这些抗体反应，也不产生交叉反应抗体。补体蛋白和淋巴细胞PFP的同源结构域可能在其附着于脂质膜和膜损伤的组装中发挥相关功能。
Structural, functional and immunological similarities between the ninth component of complement (C9) and the lymphocyte pore-forming protein (PFP, perforin) have recently been described (8-10). PFP is shown here to be immunologically related to all other components of the membrane attack complex (MAC) of human complement, namely, C5b-6, C7, C8, and C9. Polyclonal antibodies raised against purified human C5b-6, C7, C8, or C9 react with other components of the MAC and with mouse lymphocyte PFP. The antigenic epitopes shared by human complement proteins and mouse lymphocyte PFP are limited to cysteine-rich domains. Only complement proteins that have been reduced and alkylated elicit the production of crossreactive antibodies when used as immunogens. The nonreduced forms of complement components or lymphocyte PFP neither react with these antibodies nor give rise to crossreactive antibodies. The homologous domains of complement proteins and lymphocyte PFP may play related functions in their attachment to lipid membranes and assembly of membrane lesions.
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DOI：
10.1073/pnas.83.15.5668
发表时间：
1986-08-01
影响因子：
11.1
作者：
YOUNG, JDE;LEONG, LG;COHN, ZA
通讯作者：
COHN, ZA
DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J
影响因子：
--
作者：
Campbell, R D;Carroll, M C;Porter, R R
通讯作者：
Porter, R R
DOI：
10.1073/pnas.81.23.7298
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DISCIPIO, RG;GEHRING, MR;FEY, GH
通讯作者：
FEY, GH
DOI：
10.1073/pnas.79.2.574
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
PODACK, ER;TSCHOPP, J
通讯作者：
TSCHOPP, J