Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes.
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DOI：
10.1186/s12870-016-0887-0
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发表时间：
2016-09-15
影响因子：
5.3
通讯作者：
Lahaye M
中科院分区：
文献类型：
--
作者：
Dheilly E;Gall SL;Guillou MC;Renou JP;Bonnin E;Orsel M;Lahaye M
果实品质取决于一系列的生化事件，这些事件在果实发育和成熟过程中改变了外观、风味和质地。细胞壁多糖重塑在很大程度上有助于肉质果实质地的形成。虽然已知参与细胞壁多糖生物合成和修饰的几种基因和酶，但它们在这些过程中的协调活性尚未被发现。结合转录组学和生物化学分析，可以确定参与细胞壁多糖组成和结构变化在苹果果实生长和成熟过程中的基因家族的推定酶和相关注释成员。早期发育基因主要与细胞壁的生物合成和降解有关，并以半纤维素为靶标。半乳葡甘露聚糖的精细结构演变与甘露聚糖合成酶、葡聚糖酶（GH 9）和β-半乳糖苷酶基因表达密切相关。与此相反，果胶代谢和细胞扩张（expansin基因）相关的基因较少，观察到在成熟的水果结合细胞壁多糖组合物的预期变化。半纤维素经历主要的结构变化，特别是在果实发育早期。大量早期表达的β-半乳糖苷酶基因质疑它们在果实发育和贮藏过程中对半乳糖基化结构的功能。它们的活性和细胞壁底物仍有待鉴定。此外，过氧化物酶和转运蛋白的潜在作用，沿着细胞壁代谢的新见解开辟了道路，进一步研究在果实发育和贮藏过程中细胞壁组装/拆卸的伴随机制。本文的在线版本（doi：10.1186/s12870-016-0887-0）包含补充材料，可供授权用户使用。
Fruit quality depends on a series of biochemical events that modify appearance, flavour and texture throughout fruit development and ripening. Cell wall polysaccharide remodelling largely contributes to the elaboration of fleshy fruit texture. Although several genes and enzymes involved in cell wall polysaccharide biosynthesis and modifications are known, their coordinated activity in these processes is yet to be discovered. Combined transcriptomic and biochemical analyses allowed the identification of putative enzymes and related annotated members of gene families involved in cell wall polysaccharide composition and structural changes during apple fruit growth and ripening. The early development genes were mainly related to cell wall biosynthesis and degradation with a particular target on hemicelluloses. Fine structural evolutions of galactoglucomannan were strongly correlated with mannan synthase, glucanase (GH9) and β-galactosidase gene expression. In contrast, fewer genes related to pectin metabolism and cell expansion (expansin genes) were observed in ripening fruit combined with expected changes in cell wall polysaccharide composition. Hemicelluloses undergo major structural changes particularly during early fruit development. The high number of early expressed β-galactosidase genes questions their function on galactosylated structures during fruit development and storage. Their activity and cell wall substrate remains to be identified. Moreover, new insights into the potential role of peroxidases and transporters, along with cell wall metabolism open the way to further studies on concomitant mechanisms involved in cell wall assembly/disassembly during fruit development and storage. The online version of this article (doi:10.1186/s12870-016-0887-0) contains supplementary material, which is available to authorized users.
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影响因子：
9.5
作者：
Cosgrove DJ
通讯作者：
Cosgrove DJ
影响因子：
6.9
作者：
Brummell, DA;Dal Cin, V;Labavitch, JM
通讯作者：
Labavitch, JM
影响因子：
4.3
作者：
Dunand, C;Tognolli, M;Penel, C
通讯作者：
Penel, C
影响因子：
6.9
作者：
Castellarin SD;Gambetta GA;Wada H;Krasnow MN;Cramer GR;Peterlunger E;Shackel KA;Matthews MA
通讯作者：
Matthews MA
影响因子：
7
作者：
Atkinson, Ross G.;Johnston, Sarah L.;Schroder, Roswitha
通讯作者：
Schroder, Roswitha