Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR.
Rapid detection of Pseudomonas aeruginosa from positive blood cultures by quantitative PCR.
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DOI：
10.1186/1476-0711-9-21
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发表时间：
2010-08-04
影响因子：
5.7
通讯作者：
Legrand P
中科院分区：
文献类型：
--
作者：
Cattoir V;Gilibert A;Le Glaunec JM;Launay N;Bait-Mérabet L;Legrand P
铜绿假单胞菌是许多血液感染的罪魁祸首，在最初的抗菌治疗不当的情况下，会出现严重的不良后果。本研究旨在建立一种新的以ecfX为特异性靶基因的定量聚合酶链式反应(QPCR)方法，用于从阳性血培养(BCS)中快速、准确地鉴定铜绿假单胞菌。在2008年8月至2009年6月期间，对100个革兰氏阴性杆菌阳性的BC瓶进行了检测，以常规方法(即培养和表型鉴定)为金标准来评价qPCR技术的性能。共分离出铜绿假单胞菌33株，肠杆菌属53株，嗜麦芽窄食单胞菌9株，革兰氏阴性杆菌2株。除1株铜绿假单胞菌混合培养外，其余临床分离株均可用定量聚合酶链式反应进行检测。定量聚合酶链式反应的特异度为100%，阳性预测值为100%，阴性预测值为98.5%，敏感性为97%。这项可靠的技术可能会提供一种快速(1.5小时)的工具，帮助临床医生更早地开始适当的治疗。需要进一步的研究来评估这一新策略与表型方法相比的临床益处。
Pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. The present study was aimed to develop a novel quantitative PCR (qPCR) assay, using ecfX as the specific target gene, for the rapid and accurate identification of P. aeruginosa from positive blood cultures (BCs). Over the period August 2008 to June 2009, 100 BC bottles positive for gram-negative bacilli were tested in order to evaluate performances of the qPCR technique with conventional methods as gold standard (i.e. culture and phenotypic identification). Thirty-three strains of P. aeruginosa, 53 strains of Enterobactericaeae, nine strains of Stenotrophomonas maltophilia and two other gram-negative species were isolated while 3 BCs were polymicrobial including one mixture containing P. aeruginosa. All P. aeruginosa clinical isolates were detected by qPCR except a single strain in mixed culture. Performances of the qPCR technique were: specificity, 100%; positive predictive value, 100%; negative predictive value, 98.5%; and sensitivity, 97%. This reliable technique may offer a rapid (<1.5 h) tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to phenotypic methods.
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影响因子：
11.8
作者：
Schechner, Vered;Nobre, Vandack;Carmeli, Yehuda
通讯作者：
Carmeli, Yehuda
影响因子：
11.8
作者：
Kang, CI;Kim, SH;Choe, KW
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Choe, KW
影响因子：
3
作者：
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Kibbler, CC
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作者：
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通讯作者：
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影响因子：
3.1
作者：
da Silva, LVF;Tateno, AF;Ramos, SRTS
通讯作者：
Ramos, SRTS