An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells.
An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells.
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DOI：
10.1016/j.xpro.2020.100213
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Burridge PW
中科院分区：
文献类型：
--
作者：
Lyra-Leite DM;Fonoudi H;Gharib M;Burridge PW
本文提供的方案描述了用于制备用于培养hiPSC的高度成本有效的化学成分确定的培养基的方法，我们称之为B8培养基。B8培养基的典型成本为每升10美元，经过修改后比标准培养基更实惠。我们提供了制备B8补充剂等分试样、制备基础培养基DMEM/F12、Matrigel包被板、解冻、传代、培养和冷冻保存hiPSC的简单方案。我们展示了典型的差异化结果，并提供了全面的故障排除指南。有关本方案使用和执行的完整详细信息，请参阅。该方案描述了一种用于培养hiPSC的成本有效的化学成分确定的B8培养基。B8培养基的典型成本为每升10美元，这比B8补充等分试样、基础培养基DMEM/F12和基质胶包被的板的现有培养基方案更实惠。和冷冻保存hiPSC本文提供的方案描述了用于制备用于培养hiPSC的高度成本有效的化学成分确定的培养基的方法，我们称之为B8培养基。B8培养基的典型成本为每升10美元，经过修改后比标准培养基更实惠。我们提供了制备B8补充剂等分试样、制备基础培养基DMEM/F12、Matrigel包被板、解冻、传代、培养和冷冻保存hiPSC的简单方案。我们展示了典型的差异化结果，并提供了全面的故障排除指南。
The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide. For complete details on the use and execution of this protocol, please refer to. The protocol describes a cost-effective, chemically defined B8 medium for culturing hiPSCs The typical cost for B8 medium is US$10 per liter, which is more affordable than existing media Protocols for B8 supplement aliquots, basal media DMEM/F12, and Matrigel-coated plates We also describe the process of thawing, passaging, culturing, and cryopreserving hiPSCs The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple protocols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide.
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影响因子：
48
作者：
Burridge, Paul W.;Matsa, Elena;Shukla, Praveen;Lin, Ziliang C.;Churko, Jared M.;Ebert, Antje D.;Lan, Feng;Diecke, Sebastian;Huber, Bruno;Mordwinkin, Nicholas M.;Plews, Jordan R.;Abilez, Oscar J.;Cui, Bianxiao;Gold, Joseph D.;Wu, Joseph C.
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5.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
16.6
作者：
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通讯作者：
Cuppen, Edwin