Expression of CD74 in bladder cancer and its suppression in association with cancer proliferation, invasion and angiogenesis in HT-1376 cells.
Expression of CD74 in bladder cancer and its suppression in association with cancer proliferation, invasion and angiogenesis in HT-1376 cells.
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DOI：
10.3892/ol.2018.8309
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发表时间：
2018-05
期刊：
影响因子：
2.9
通讯作者：
Xing N
中科院分区：
文献类型：
--
作者：
Gai JW;Wahafu W;Song L;Ping H;Wang M;Yang F;Niu Y;Qing W;Xing N
本研究的目的是研究CD 74在人膀胱尿路上皮细胞癌（UCB）中的表达及其可能的作用。CD 74和巨噬细胞移动抑制因子（MIF）的定位和测定在正常和UCB样品和细胞系使用免疫染色。在HT-1376细胞中使用CD 74 shRNA慢病毒颗粒敲低CD 74。检测CD 74基因敲低的HT-1376细胞的增殖、侵袭能力和微血管密度（MVD）。还在体内验证了CD 74在另外的高级别UCB J82细胞系中的表达。所有实验重复至少3次。与正常的非肌肉浸润性膀胱癌（NMIBC）样本和其他细胞系相比，大多数肌肉浸润性膀胱癌（MIBC）样本和仅一种高级别UCB细胞系HT-1376表达CD 74。CD 74基因敲低的HT-1376细胞的增殖和侵袭能力明显降低，Western blotting结果显示，不同的体外处理对HT-1376细胞增殖、凋亡和侵袭相关蛋白的表达水平有不同程度的影响。肿瘤发生和MVD检测表明，与scramble细胞相比，敲低的HT-1376细胞的增殖和血管生成较少。值得注意的是，在体外不显示CD 74信号的J82细胞在体内呈现CD 74的表达。本研究揭示了CD 74在MIBC的增殖、侵袭和血管生成中的潜在作用，并且其可能作为UCB的潜在治疗靶点，但需要进一步的研究。
The aim of the present study was to investigate the expression and potential roles of CD74 in human urothelial cell carcinoma of the bladder (UCB) in vitro and in vivo. CD74 and macrophage migration inhibitory factor (MIF) were located and assayed in normal and UCB samples and cell lines using immunostaining. CD74 was knocked down using CD74 shRNA lentiviral particles in HT-1376 cells. The proliferative, invasive potential and microvessel density (MVD) of knockdown-CD74 HT-1376 cells were analyzed in vitro or in vivo. The expression of CD74 in an additional high grade UCB J82 cell line was also verified in vivo. All experiments were repeated at least 3 times. The majority of muscle-invasive bladder cancer (MIBC) samples, and only one high grade UCB cell line, HT-1376, expressed CD74, compared with normal, non-muscle-invasive bladder cancer (NMIBC) samples and other cell lines. The levels of proliferation and invasion were decreased in the CD74 knockdown-HT-1376 cells, and western blotting assay indicated that the levels of proteins associated with proliferation, apoptosis and invasion in the cells were affected correspondingly by different treatments in vitro. The tumorigenesis and MVD assays indicated less proliferation and angiogenesis in the knockdown-HT-1376 cells compared with the scramble cells. Notably, J82 cells exhibiting no signal of CD74 in vitro presented the expression of CD74 in vivo. The present study revealed the potential roles of CD74 in the proliferation, invasion and angiogenesis of MIBC, and that it may serve as a potential therapeutic target for UCB, but additional studies are required.
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影响因子：
20.3
作者：
Starlets, Diana;Gore, Yael;Shachar, Idit
通讯作者：
Shachar, Idit
DOI：
10.1016/j.urolonc.2010.04.008
发表时间：
2010-07
期刊：
Urologic oncology
影响因子：
--
作者：
McConkey DJ;Lee S;Choi W;Tran M;Majewski T;Lee S;Siefker-Radtke A;Dinney C;Czerniak B
通讯作者：
Czerniak B
影响因子：
2.6
作者：
Choi, Jung-Woo;Kim, Younghye;Kim, Young-Sik
通讯作者：
Kim, Young-Sik
影响因子：
6.1
作者：
Cao, WG;Morin, M;Akoum, A
通讯作者：
Akoum, A
DOI：
10.1097/igc.0b013e31821c45b7
发表时间：
2011-08-01
影响因子：
4.8
作者：
Cheng, Rong-jie;Deng, Wei-guo;Fu, Yan
通讯作者：
Fu, Yan