Saturation effects on ultraviolet resonance Raman intensities : excimer/YAG laser comparison and aromatic amino acid cross-sections
Saturation effects on ultraviolet resonance Raman intensities : excimer/YAG laser comparison and aromatic amino acid cross-sections
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饱和度对紫外共振拉曼强度的影响：准分子/YAG 激光比较和芳香族氨基酸横截面
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
T. Spiro
中科院分区：
文献类型：
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作者：
C. Su;Yang Wang;T. Spiro
用H2拉曼位移YAG或倍频准分子泵浦染料激光器激发细胞色素c中芳香族残基（230 nm）的共振拉曼（RR）带，观察到大量的饱和效应。在给定的平均功率下，准分子的饱和比YAG激发低得多，因为准分子激光的脉冲更长，重复率更高。在低平均功率下，准分子激发光谱的信号质量显著提高。在240 ~ 192nm波长范围内，重新测定了苯丙氨酸、酪氨酸和色氨酸的紫外反射光谱横截面。准分子激光用于209 nm及更长的波长，此外，反褶积技术应用于更好地定义单个RR波段。这些改进导致了截面值的定量变化，但从芳香残基激发态性质的角度解释激发曲线保持不变。
Substantial saturation effects are noted for resonance Raman (RR) bands of the aromatic residues in cytochrome c (excited at 230 nm) with an H2 Raman-shifted YAG or a frequency-doubled excimer-pumped dye laser. At a given average power, saturation is much lower for excimer than for YAG excitation because of the longer pulse and higher repetition rate of the excimer laser. The signal quality at low average power is significantly higher for the excimer-excited spectra. Ultraviolet RR cross-sections have been redetermined for aqueous phenylalanine, tyrosine and tryptophan at a series of wavelengths from 240 to 192 nm. The excimer laser was used at 209 nm and longer wavelengths, and in addition deconvolution techniques were applied to better define the individual RR bands. These improvements led to quantitative changes in the cross-section values from those reported previously, but the interpretation of the excitation profiles in terms of excited-state properties of the aromatic residues remains unchanged.
紫外共振拉曼光谱的细胞色素 c 结构和 Trp-59 H/D 交换的离子强度依赖性。
影响因子：
2.9
作者：
Liu,GY;Grygon,CA;Spiro,TG
通讯作者：
Spiro,TG
影响因子：
2.9
作者：
HILDEBRANDT, PG;COPELAND, RA;PRENDERGAST, FG
通讯作者：
PRENDERGAST, FG
通过深（192-223 nm）紫外拉曼光谱测定蛋白质的二级结构。
影响因子：
2.9
作者：
Copeland,RA;Spiro,TG
通讯作者：
Spiro,TG