Role of visible light-activated photocatalyst on the reduction of anthrax spore-induced mortality in mice.
Role of visible light-activated photocatalyst on the reduction of anthrax spore-induced mortality in mice.
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DOI：
10.1371/journal.pone.0004167
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Chang HH
中科院分区：
文献类型：
--
作者：
Kau JH;Sun DS;Huang HH;Wong MS;Lin HC;Chang HH
二氧化钛（TiO 2）基底的光催化作用主要由紫外光照射引起。阴离子掺杂的TiO 2在可见光照射下表现出光催化活性，是一种相对环境友好的材料。然而，它们对炭疽杆菌的抗孢子活性仍有待研究。我们评估了这些可见光激活的光催化剂对减少炭疽孢子诱导的发病机制。采用标准平板法测定可见光诱导灭活炭疽芽孢的效果。进一步采用小鼠模型来研究该化合物对炭疽毒素和孢子介导的死亡率的抑制作用。我们发现，可见光照射氮或碳掺杂的二氧化钛薄膜的抗孢子活动显着降低炭疽孢子的生存能力。尽管孢子杀灭效率仅为约25%，但我们的数据表明，来自光催化组而非未处理组的孢子在巨噬细胞清除后具有较低的存活率。此外，该菌还能直接抑制B的主要毒力因子--致死毒素。炭疽病与这些结果一致，我们发现光催化孢子诱导小鼠死亡的效力低十倍。这些数据表明，芽孢杆菌可能通过灭活孢子组分来损伤孢子。光催化对孢子的损伤可能比直接杀灭孢子更能降低病原菌对寄主的致病性。
Photocatalysis of titanium dioxide (TiO2) substrates is primarily induced by ultraviolet light irradiation. Anion-doped TiO2 substrates were shown to exhibit photocatalytic activities under visible-light illumination, relative environmentally-friendly materials. Their anti-spore activity against Bacillus anthracis, however, remains to be investigated. We evaluated these visible-light activated photocatalysts on the reduction of anthrax spore-induced pathogenesis. Standard plating method was used to determine the inactivation of anthrax spore by visible light-induced photocatalysis. Mouse models were further employed to investigate the suppressive effects of the photocatalysis on anthrax toxin- and spore-mediated mortality. We found that anti-spore activities of visible light illuminated nitrogen- or carbon-doped titania thin films significantly reduced viability of anthrax spores. Even though the spore-killing efficiency is only approximately 25%, our data indicate that spores from photocatalyzed groups but not untreated groups have a less survival rate after macrophage clearance. In addition, the photocatalysis could directly inactivate lethal toxin, the major virulence factor of B. anthracis. In agreement with these results, we found that the photocatalyzed spores have tenfold less potency to induce mortality in mice. These data suggest that the photocatalysis might injury the spores through inactivating spore components. Photocatalysis induced injuries of the spores might be more important than direct killing of spores to reduce pathogenicity in the host.
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影响因子：
2.6
作者：
Kau, JH;Lin, CG;Lin, HC
通讯作者：
Lin, HC
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