Convergent and parallel activation of low-conductance potassium channels by calcium and cAMP-dependent protein kinase.
Convergent and parallel activation of low-conductance potassium channels by calcium and cAMP-dependent protein kinase.
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钙和 cAMP 依赖性蛋白激酶对低电导钾通道的收敛和并行激活。
DOI：
10.1073/pnas.92.15.7115
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发表时间：
1995
影响因子：
11.1
通讯作者：
Lidofsky,SD
中科院分区：
文献类型：
--
作者：
Lidofsky,SD
K+ channels, which have been linked to regulation of electrogenic solute transport as well as Ca2+ influx, represent a locus in hepatocytes for the concerted actions of hormones that employ Ca2+ and cAMP as intracellular messengers. Despite considerable study, the single-channel basis for synergistic effects of Ca2+ and cAMP on hepatocellular K+ conductance is not well understood. To address this question, patch-clamp recording techniques were applied to a model liver cell line, HTC hepatoma cells. Increasing the cytosolic Ca2+ concentration ([Ca2+]i) in HTC cells, either by activation of purinergic receptors with ATP or by inhibition of intracellular Ca2+ sequestration with thapsigargin, activated low-conductance (9-pS) K+ channels. Studies with excised membrane patches suggested that these channels were directly activated by Ca2+. Exposure of HTC cells to a permeant cAMP analog, 8-(4-chlorophenylthio)-cAMP, also activated 9-pS K+ channels but did not change [Ca2+]i. In excised membrane patches, cAMP-dependent protein kinase (the downstream effector of cAMP) activated K+ channels with conductance and selectivity identical to those of channels activated by Ca2+. In addition, cAMP-dependent protein kinase activated a distinct K+ channel type (5 pS). These data represent the differential regulation of low-conductance K+ channels by signaling pathways mediated by Ca2+ and cAMP. Moreover, since low-conductance Ca(2+)-activated K+ channels have been identified in a variety of cell types, these findings suggest that differential regulation of K+ channels by hormones with distinct signaling pathways may provide a mechanism for hormonal control of solute transport and Ca(2+)-dependent cellular functions in the liver as well as other nonexcitable tissues.
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DOI：
10.1152/ajpgi.1993.264.3.g478
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Lidofsky,SD;Fitz,JG;Weisiger,RA;Scharschmidt,BF
通讯作者：
Scharschmidt,BF
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DOI：
10.1152/ajpgi.1994.266.4.g677
发表时间：
1994
期刊：
The American journal of physiology
影响因子：
--
作者：
Fitz,JG;Sostman,AH;Middleton,JP
通讯作者：
Middleton,JP
影响因子：
64.5
作者：
LENK, R;RANSOM, L;PENMAN, S
通讯作者：
PENMAN, S
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DOI：
10.1152/ajplung.1990.258.6.l334
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
McCann,JD;Matsuda,J;Garcia,M;Kaczorowski,G;Welsh,MJ
通讯作者：
Welsh,MJ
豚鼠肝细胞中增加胞质钙和环磷酸腺苷的受体之间的相互作用
影响因子：
7.3
作者：
T. Cocks;D. Jenkinson;K. Koller
通讯作者：
K. Koller